Social Isolation Induces Sex-Specific Differences in Behavior and Gut Microbiota Composition in Stress-Sensitive Rats

IF 2.6 3区 心理学 Q2 BEHAVIORAL SCIENCES
Charlotte Hurst, Gosia Zobel, Wayne Young, Trent Olson, Nabil Parkar, Jeremy Bracegirdle, Rina Hannaford, Rachel C. Anderson, Julie E. Dalziel
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引用次数: 0

Abstract

Background

Social isolation (SI) is an established rat model of chronic stress. We applied this to the stress-sensitive Wistar Kyoto (WKY) strain to explore brain-to-gut interactions associated with mood. Whether SI stress-induced behavioral changes are sex-specific or if they affect the microbiome in WKY is unknown. We hypothesized individually housed (IH) animals would be more anxious than pair-housed (PH), with sex differences. Male and female rats were either IH or PH from 70 to 112 days old and behavior was assessed in modified open field (OFTmod), elevated plus maze (EPM), and novel object recognition (NOR) tests. Cecal content DNA was analyzed by shotgun metagenome sequencing.

Results

IH rats, particularly females, spent more time in the center of the OFTmod where the semi-novel feed was presented compared to PH group rats. There was a tendency for greater distance traveled, or potential hyperactivity, in IH female rats. Males stayed in the EPM closed arms more than females. No treatment difference occurred for recognition memory. SI altered cecal microbiome composition in females where housing was associated with seven differentially abundant taxa and 49 differentially abundant KEGG Level 3 ortholog/gene categories. Several relationships were noted between behavioral traits and relative abundance of microbiome taxa. There was a greater shift in female microbiome composition.

Conclusions

In summary, behavioral responses to the housing treatment were minimal. IH animals, particularly females, spent more time in the center of an OFT that contained food; this may have been an indication of depression, as opposed to anxiety. Housing status had a differential impact on the microbiome for females compared to males. The associations between cecal microbiota and activity in the modified OFT suggest that dietary interventions that influence the relative abundance of Bifidobacteria, Alistipes, and Muribaculaceae should be explored.

Abstract Image

社会隔离诱导应激敏感大鼠行为和肠道微生物群组成的性别特异性差异
社会隔离(Social isolation, SI)是一种已建立的大鼠慢性应激模型。我们将此应用于对压力敏感的Wistar Kyoto (WKY)菌株,以探索与情绪相关的脑-肠相互作用。SI应激诱导的行为改变是否具有性别特异性,或者是否会影响WKY中的微生物组尚不清楚。我们假设单独饲养(IH)的动物比成对饲养(PH)的动物更焦虑,存在性别差异。雄性和雌性大鼠在70至112日龄时分别进行IH或PH测试,并通过改良开阔场(OFTmod)、升高加迷宫(EPM)和新物体识别(NOR)测试评估其行为。盲肠内容DNA采用散弹枪宏基因组测序分析。结果与PH组大鼠相比,IH组大鼠,尤其是雌性大鼠,在喂食半新颖饲料的OFTmod中心停留的时间更长。在IH雌性大鼠中,有更大的行走距离或潜在的多动倾向。男性在EPM中闭臂的时间比女性更长。两组在识别记忆方面无显著差异。SI改变了雌性盲肠微生物组的组成,其中住房与7个差异丰富的分类群和49个差异丰富的KEGG 3级同源/基因类别相关。行为特征与微生物类群的相对丰度之间存在一些关系。雌性微生物组组成的变化更大。综上所述,对住房治疗的行为反应最小。IH动物,尤其是雌性,在有食物的OFT中心呆的时间更长;这可能是抑郁的迹象,而不是焦虑。与男性相比,住房状况对女性微生物组的影响有所不同。盲肠菌群与改良后的OFT中活性之间的关系表明,应该探索影响双歧杆菌、阿里斯特氏菌和Muribaculaceae相对丰度的饮食干预。
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来源期刊
Brain and Behavior
Brain and Behavior BEHAVIORAL SCIENCES-NEUROSCIENCES
CiteScore
5.30
自引率
0.00%
发文量
352
审稿时长
14 weeks
期刊介绍: Brain and Behavior is supported by other journals published by Wiley, including a number of society-owned journals. The journals listed below support Brain and Behavior and participate in the Manuscript Transfer Program by referring articles of suitable quality and offering authors the option to have their paper, with any peer review reports, automatically transferred to Brain and Behavior. * [Acta Psychiatrica Scandinavica](https://publons.com/journal/1366/acta-psychiatrica-scandinavica) * [Addiction Biology](https://publons.com/journal/1523/addiction-biology) * [Aggressive Behavior](https://publons.com/journal/3611/aggressive-behavior) * [Brain Pathology](https://publons.com/journal/1787/brain-pathology) * [Child: Care, Health and Development](https://publons.com/journal/6111/child-care-health-and-development) * [Criminal Behaviour and Mental Health](https://publons.com/journal/3839/criminal-behaviour-and-mental-health) * [Depression and Anxiety](https://publons.com/journal/1528/depression-and-anxiety) * Developmental Neurobiology * [Developmental Science](https://publons.com/journal/1069/developmental-science) * [European Journal of Neuroscience](https://publons.com/journal/1441/european-journal-of-neuroscience) * [Genes, Brain and Behavior](https://publons.com/journal/1635/genes-brain-and-behavior) * [GLIA](https://publons.com/journal/1287/glia) * [Hippocampus](https://publons.com/journal/1056/hippocampus) * [Human Brain Mapping](https://publons.com/journal/500/human-brain-mapping) * [Journal for the Theory of Social Behaviour](https://publons.com/journal/7330/journal-for-the-theory-of-social-behaviour) * [Journal of Comparative Neurology](https://publons.com/journal/1306/journal-of-comparative-neurology) * [Journal of Neuroimaging](https://publons.com/journal/6379/journal-of-neuroimaging) * [Journal of Neuroscience Research](https://publons.com/journal/2778/journal-of-neuroscience-research) * [Journal of Organizational Behavior](https://publons.com/journal/1123/journal-of-organizational-behavior) * [Journal of the Peripheral Nervous System](https://publons.com/journal/3929/journal-of-the-peripheral-nervous-system) * [Muscle & Nerve](https://publons.com/journal/4448/muscle-and-nerve) * [Neural Pathology and Applied Neurobiology](https://publons.com/journal/2401/neuropathology-and-applied-neurobiology)
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