CRISPR/Cas-based SERS detection: A win-win integration towards real-world applications

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-06-06 DOI:10.1016/j.trac.2025.118334

Ruibo Zhao , Yaru Li , Xinyue Li , Myunghee Kim , Xingjie Li , Shichun Pei , Shuli Man , Weipan Peng , Long Ma

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引用次数: 0

Abstract

In recent years, the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated nuclease (Cas) system has risen as a powerful tool for genome editing. Beyond its editing capabilities, CRISPR/Cas system has garnered significant interest in molecular diagnostics, especially for nucleic acid detection due to its high sensitivity and specificity. Surface-enhanced Raman spectroscopy (SERS), reliant on plasmonic nanoparticles or nanostructures, was extensively employed in biosensing owing to its exceptional sensitivity and distinctive spectral bands. The integration of SERS with CRISPR/Cas technology led to the development of numerous biosensing approaches aimed at achieving ultra-sensitive detection. This review provided an overview of both the CRISPR/Cas and SERS technology, followed by a comprehensive summary of their combined application for nucleic acid detection. Furthermore, it delved into the current landscape of CRISPR/Cas-based SERS detection, addressing existing challenges and prospects for future advancements.

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基于CRISPR/ cas的SERS检测：与现实世界应用的双赢整合

近年来，集群规则间隔短回文重复序列（CRISPR）相关核酸酶（Cas）系统已成为基因组编辑的有力工具。除了编辑能力之外，CRISPR/Cas系统还因其高灵敏度和特异性而在分子诊断领域引起了极大的兴趣，特别是在核酸检测领域。表面增强拉曼光谱（SERS），依赖于等离子体纳米粒子或纳米结构，由于其卓越的灵敏度和独特的光谱带被广泛应用于生物传感。SERS与CRISPR/Cas技术的整合导致了许多旨在实现超灵敏检测的生物传感方法的发展。本文综述了CRISPR/Cas和SERS技术，并对它们在核酸检测中的联合应用进行了综述。此外，它还深入研究了基于CRISPR/ cas的SERS检测的现状，解决了现有的挑战和未来进展的前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.