Lasiodiplodia theobromae, Pestalotiopsis clavispora and Fusarium spp. associated with pomegranate dieback and fruit rot in Tunisia

IF 2.5 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2025-06-09 DOI:10.1016/j.cropro.2025.107310

Hayfa Jabnoun-Khiareddine , Rania Aydi Ben Abdallah , Messaoud Mars , Mejda Daami-Remadi

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引用次数: 0

Abstract

Tunisia is one of the main pomegranate (Punica granatum L.) producing countries. Between 2018 and 2021, a 15–20 % incidence of dieback and fruit rot was observed in many pomegranate orchards along Tunisian coasts. Faced with this issue, the current study aimed to characterize the etiological agents associated with these symptoms. Morphological characterization combined with multigene-combined phylogenetic analyses revealed that five fungal species namely Lasiodiplodia theobromae, Neopestalotiopsis clavispora, Fusarium equiseti, F. brachygibbosum, and F. incarnatum were involved in branch dieback, while L. theobromae and F. proliferatum species were associated with pomegranate fruit rot symptoms. As far as our knowledge extends, this is the first report of the involvement of these fungal species in pomegranate dieback and fruit rot in Tunisia and in Africa. Pathogenicity tests carried out through inoculation of detached pomegranate cv. Gabsi shoots and fruits showed that all tested fungal species were pathogenic by inducing disease symptoms with different degrees of severity. Given the rapid and severe progress of these diseases, it is critical to implement prevention and management strategies to alleviate their incidence and reduce tree losses.

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突尼斯石榴枯萎病和果实腐病的相关病原菌：茶枯病、拟盘多毛孢和镰刀菌

突尼斯是石榴（石榴）的主要生产国之一。在2018年至2021年期间，突尼斯沿海的许多石榴果园中观察到15 - 20%的枯萎和果实腐烂发生率。面对这一问题，目前的研究旨在描述与这些症状相关的病因。形态学鉴定结合多基因联合系统发育分析发现，5种真菌分别为Lasiodiplodia theobromae、Neopestalotiopsis clavispora、Fusarium equiseti、F. brachygibbosum和F. incarnatum与枝条枯死有关，而L. theobromae和F. proliferatum与石榴果实腐烂症状有关。据我们所知，这是首次报道这些真菌物种在突尼斯和非洲的石榴枯死和果实腐烂中存在。通过离体石榴cv接种进行了致病性试验。通过诱导不同程度的疾病症状，表明所有被试真菌都具有致病性。鉴于这些疾病的迅速和严重进展，实施预防和管理战略以减轻其发病率和减少树木损失至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.