Decoding the fate of antibiotic resistance genes in soil with the application of thermally treated gentamicin mycelial residues

Q1 Environmental Science

Bioresource Technology Reports Pub Date : 2025-06-01 DOI:10.1016/j.biteb.2025.102169

Dong Wang , Qingwen Zhang , Zhihao Sun , Yuefei Li , Xiaomei Xie , Shuaishuai Xin , Guocheng Liu , Huiling Liu , Yanjun Xin

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Abstract

Gentamicin mycelium residues (GMRs) is a type of organic waste generated during the production of gentamicin. Although thermal treatment could eliminate the majority of antibiotic in GMRs, the resistance risks caused by residual gentamicin still require further investigation. This study aimed to evaluate the resistance risks of thermal-treated GMRs as a soil amendment by monitoring changes in gentamicin, resistance genes and bacterial community in the soil. The results indicated that gentamicin could be rapidly degraded in soil amended with thermal-treated GMRs (TGMRs) within 1st day, the abundance of AAC(6′)-Ie-APH (2′)-Ia, armA, APH(3′)-VI, ANT(2″)-Ia, Tn916/1545 and intIl restored to the soil background level at the 90th day. Furthermore, TGMRs could increase the abundance of soil bacteria that were beneficial for organic matter decomposition, while reducing the adverse effects of gentamicin on bacterial community diversity and richness. These findings provided more references for the environmental risk management of TGMRs in agricultural applications.

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利用热处理庆大霉素菌丝残留物破解土壤中抗生素耐药基因的命运

庆大霉素菌丝残留物（GMRs）是庆大霉素生产过程中产生的一种有机废物。虽然热处理可以消除gmr中的大部分抗生素，但残留庆大霉素引起的耐药风险仍需进一步研究。本研究旨在通过监测土壤中庆大霉素、耐药基因和细菌群落的变化，评估热处理gmr作为土壤改良剂的耐药风险。结果表明：庆大霉素在经过热处理的GMRs （TGMRs）改性的土壤中可在第1天内迅速降解，第90天AAC（6’）-Ie-APH（2’）-Ia、armA、APH（3’）-VI、ANT（2″）-Ia、Tn916/1545和intIl丰度恢复到土壤背景水平。此外，tgmr可以增加有利于有机质分解的土壤细菌的丰度，同时减少庆大霉素对细菌群落多样性和丰富度的不利影响。研究结果可为tgmr在农业应用中的环境风险管理提供参考。

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