β-Galactosidase-activated bioluminescent probe for in vivo tumor imaging†

IF 4.2 2区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY
Tao Hu , Yuexia Yang , Bijia Zhou , Zhaoming Chen , Fapu Wu , Hu Xiong
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引用次数: 0

Abstract

β-Galactosidase (GAL) is a lysosomal protease implicated in cellular senescence and various malignant cancers. However, bioluminescent probes for specific GAL detection remain scarce, and their applications are largely limited to transgenic models expressing firefly luciferase (fLuc). Herein, we report a bioluminescent probe, , for detecting GAL activity in nontransgenic cells and tumor-bearing mice. exhibited high selectivity toward GAL, with a limit of detection of 8.5 mU mL−1. Combined with fLuc mRNA-loaded lipid nanoparticles, was able to sensitively monitor GAL activity in vitro and in vivo. Based on this strategy, intestinal metastatic tumors were precisely delineated, showing 25.2-fold higher bioluminescence intensity in non-transgenic tumor-bearing mice.

Abstract Image

用于体内肿瘤成像的β-半乳糖苷酶激活生物发光探针
β-半乳糖苷酶(GAL)是一种溶酶体蛋白酶,与细胞衰老和各种恶性癌症有关。然而,用于特异性GAL检测的生物发光探针仍然很少,它们的应用很大程度上局限于表达萤火虫荧光素酶(fLuc)的转基因模型。在此,我们报道了一种生物发光探针β-Luc,用于检测非转基因细胞和荷瘤小鼠中的GAL活性。β-Luc对GAL具有较高的选择性,检出限为8.5 mU/mL。与负载fLuc mrna的脂质纳米颗粒结合,β-Luc能够在体外和体内灵敏地监测GAL活性。基于这一策略,肠道转移肿瘤被精确描绘,在非转基因肿瘤小鼠中显示出高25.2倍的生物发光强度。
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来源期刊
Chemical Communications
Chemical Communications 化学-化学综合
CiteScore
8.60
自引率
4.10%
发文量
2705
审稿时长
1.4 months
期刊介绍: ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.
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