A transcriptional recognition site within SOS1 coding region controls salt tolerance in Arabidopsis

IF 10.7 1区生物学 Q1 CELL BIOLOGY

Developmental cell Pub Date : 2025-06-10 DOI:10.1016/j.devcel.2025.05.010

Kai-Kai Lu, Hong Yang, Cai-Yi Liao, Ru-Feng Song, Xiao-Yu Hu, Feng Ren, Wen-Cheng Liu

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引用次数: 0

Abstract

Salt stress impacts plant growth and development, threatening agricultural production. The Na⁺/H⁺ antiporter SALT OVERLY SENSITIVE 1 (SOS1) functions in cellular ion homeostasis through facilitating Na⁺ excretion and is therefore essential for plant salt tolerance. Here, we report that the transcription factors AUXIN RESPONSE FACTOR 7 (ARF7) and ARF19 are required for salt-induced SOS1 expression and salt tolerance in Arabidopsis thaliana. ARF7 and ARF19 activate SOS1 transcription by binding to SOS1 coding region rather than its promoter. Additionally, an E3 ubiquitin ligase, CHY ZINC-FINGER AND RING PROTEIN 1 (CHYR1), interacts with and degrades ARF7 and ARF19, dampening SOS1 expression. Upon high salinity, CHYR1 expression is inhibited in plants, stabilizing ARF7 and ARF19 proteins and increasing SOS1 expression. Collectively, our study identifies a transcriptional cis-element within SOS1 coding region recognized by ARF7 and ARF19 and elucidates a molecular mechanism governing ARF7 and ARF19 protein stability and SOS1 expression during plant salt stress response.

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SOS1编码区的转录识别位点控制拟南芥的耐盐性

盐胁迫影响植物生长发育，威胁农业生产。Na+/H+反转运蛋白SALT oversensitive 1 （SOS1）通过促进Na+的排泄，在细胞离子稳态中起作用，因此对植物耐盐性至关重要。本文中，作者报道了拟南芥盐诱导SOS1的表达和耐盐性需要转录因子AUXIN RESPONSE FACTOR 7 （ARF7）和ARF19。ARF7和ARF19通过结合SOS1编码区而不是其启动子来激活SOS1转录。此外，E3泛素连接酶CHY锌指环蛋白1 （CHYR1）与ARF7和ARF19相互作用并降解，抑制SOS1的表达。在高盐度环境下，植物体内CHYR1的表达受到抑制，ARF7和ARF19蛋白稳定，SOS1表达增加。总的来说，我们的研究在SOS1编码区发现了ARF7和ARF19识别的转录顺式元件，并阐明了植物盐胁迫响应中控制ARF7和ARF19蛋白稳定性和SOS1表达的分子机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Developmental cell 生物-发育生物学

CiteScore

18.90

自引率

1.70%

发文量

203

审稿时长

3-6 weeks

期刊介绍： Developmental Cell, established in 2001, is a comprehensive journal that explores a wide range of topics in cell and developmental biology. Our publication encompasses work across various disciplines within biology, with a particular emphasis on investigating the intersections between cell biology, developmental biology, and other related fields. Our primary objective is to present research conducted through a cell biological perspective, addressing the essential mechanisms governing cell function, cellular interactions, and responses to the environment. Moreover, we focus on understanding the collective behavior of cells, culminating in the formation of tissues, organs, and whole organisms, while also investigating the consequences of any malfunctions in these intricate processes.