Zoonotic arbovirus infections in cattle in Mozambique with special reference to Crimean-Congo hemorrhagic fever virus (CCHFV) and rift valley fever virus (RVFV).
José Fafetine, Teresa Cuinhane, Balal Sadeghi, Regina D Miambo, Lucinda de Araújo, Martin H Groschup, Ansgar Schulz
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引用次数: 0
Abstract
Background: Arboviruses pose a great threat to public health in sub-Saharan African countries. Mozambique is located in a region that is prone to climate change-related devastation, including heavy rainfalls and severe droughts that favor the emergence of zoonotic viruses transmitted by arthropods such as Crimean-Congo hemorrhagic fever virus (Orthonairovirus haemorrhagiae, CCHFV) and Rift Valley fever virus (Phlebovirus riftense, RVFV). Both viruses are closely associated with livestock farming, including cattle, and can cause symptoms of hemorrhagic fever in humans. Available previous data sets related to the presence of RVFV and especially CCHFV in Mozambique are rather scarce. Hence, the objective of this study was to evaluate the recent seroprevalence of both viruses in cattle in four localities of Limpopo National Park. In addition, ticks were collected and tested for the presence of different arboviruses.
Methodology: A total of 460 cattle blood samples were collected and analyzed for the presence of CCHFV and RVFV antibodies using ID Screen CCHF Double Antigen Multi-species (IgM/IgG) and ID Screen Rift Valley Fever Competition Multi-species commercial ELISA test kits (IDvet, Grabels, France), respectively. 1176 ticks were collected from the same animals and analyzed with different RT-qPCRs assays for CCHFV, Nairobi sheep disease virus (Orthonairovirus nairobiense, NSDV) virus and Dugbe virus (Orthonairovirus dugbeense, DUGV). Selected ticks were further screened by using a pan-Flavivirus melting curve PCR.
Results: The overall seroprevalence was higher for CCHFV (50%) compared to RVFV (28%). While a significant difference in seroprevalence between age groups was only found for CCHFV, there was a difference in RVFV seroprevalence between sampling sites that was not observed for CCHFV. None of the viruses tested were found inside the ticks.
Conclusions: This study revealed the presence of anti-CCHFV and anti-RVFV antibodies in cattle from all four sampled localities suggesting that both viruses are circulating in cattle and may be an important cause of unidentified febrile illness in humans in the region.
期刊介绍：
Virology Journal is an open access, peer reviewed journal that considers articles on all aspects of virology, including research on the viruses of animals, plants and microbes. The journal welcomes basic research as well as pre-clinical and clinical studies of novel diagnostic tools, vaccines and anti-viral therapies.
The Editorial policy of Virology Journal is to publish all research which is assessed by peer reviewers to be a coherent and sound addition to the scientific literature, and puts less emphasis on interest levels or perceived impact.
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