Two novel polyvalent phages: a promising approach for cross-order pathogen control in aquaculture.

IF 4 3区 医学 Q2 VIROLOGY
Chengcheng Li, Yufei Yue, Rui Yin, Jiulong Zhao, Zengmeng Wang, Shailesh Nair, Yongyu Zhang
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引用次数: 0

Abstract

Bacteriophages represent a promising alternative to antibiotics for controlling bacterial pathogens. However, phage application is often hindered by its narrow host range in preventing diseases caused by multiple unknown pathogens. While broad-host-range phages capable of cross-genus or cross-order infections, offer significant advantages in addressing this challenge, they are rarely isolated. In this study, we isolated two polyvalent lytic phages, SA-P and SA-M, through a multi-host enrichment strategy. These phages exhibited remarkable cross-order infectivity against the co-occurring aquaculture pathogens Shewanella algae and multiple Vibrio species. We confirmed that SA-P executes a complete lytic cycle in these cross-order hosts, indicating exceptional compatibility of its lysis systems across taxonomic orders. Genomic analysis revealed that their broad host recognition ability may stem from their diverse tail fiber and tailspike proteins. Notably, SA-P and SA-M are the first phages reported to infect S. algae, and their combined application exhibited a sustained suppression of pathogen growth. Proteomic phylogenetic analysis suggests these phages represent a novel unclassified viral genus and family, respectively. This study provides two promising polyvalent phages and their cocktails as potential solution for cross-order pathogen control in aquaculture.

两种新型多价噬菌体:一种有前途的水产养殖跨阶病原菌控制方法。
噬菌体是一种很有前途的替代抗生素来控制细菌病原体。然而,噬菌体在预防多种未知病原体引起的疾病方面的应用往往受到其宿主范围狭窄的阻碍。虽然能够跨属或跨目感染的广泛宿主噬菌体在应对这一挑战方面具有显著优势,但它们很少被分离出来。在本研究中,我们通过多宿主富集策略分离了两种多价裂解噬菌体SA-P和SA-M。这些噬菌体对共生的水产养殖病原菌希瓦氏藻和多种弧菌具有显著的跨阶感染性。我们证实了SA-P在这些跨阶宿主中执行完整的裂解循环,表明其裂解系统在不同分类顺序中具有特殊的兼容性。基因组分析表明,它们广泛的宿主识别能力可能源于它们多样的尾纤维和尾刺蛋白。值得注意的是,SA-P和SA-M是第一个被报道感染S. algae的噬菌体,它们的联合应用显示出对病原体生长的持续抑制。蛋白质组学系统发育分析表明,这些噬菌体分别代表了一个新的未分类的病毒属和科。本研究提供了两种有前途的多价噬菌体及其鸡尾酒作为水产养殖跨序病原菌控制的潜在解决方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Virology Journal
Virology Journal 医学-病毒学
CiteScore
7.40
自引率
2.10%
发文量
186
审稿时长
1 months
期刊介绍: Virology Journal is an open access, peer reviewed journal that considers articles on all aspects of virology, including research on the viruses of animals, plants and microbes. The journal welcomes basic research as well as pre-clinical and clinical studies of novel diagnostic tools, vaccines and anti-viral therapies. The Editorial policy of Virology Journal is to publish all research which is assessed by peer reviewers to be a coherent and sound addition to the scientific literature, and puts less emphasis on interest levels or perceived impact.
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