Limited passage and functional effects of polystyrene micro- and nanoplastics in a physiologically-relevant in vitro human placental co-culture model

IF 2.8 4区 医学 Q2 REPRODUCTIVE BIOLOGY
Jeske van Boxel , Sandra M. Nijmeijer , Manuel T. Heinzelmann , Sebastian Rupp , Majorie B.M. van Duursen
{"title":"Limited passage and functional effects of polystyrene micro- and nanoplastics in a physiologically-relevant in vitro human placental co-culture model","authors":"Jeske van Boxel ,&nbsp;Sandra M. Nijmeijer ,&nbsp;Manuel T. Heinzelmann ,&nbsp;Sebastian Rupp ,&nbsp;Majorie B.M. van Duursen","doi":"10.1016/j.reprotox.2025.108956","DOIUrl":null,"url":null,"abstract":"<div><div>The placenta plays a crucial role during pregnancy, yet effective <em>in vitro</em> models for placental toxicity testing are limited. In this study, a Transwell co-culture model combining BeWo b30 and HUVEC cells was developed and characterized, and used to study the transport and effects of polystyrene micro- and nanoplastics (PS-MNPs). After 72 h, 8.7 % of 50 nm and 1.2 % of 200 nm of fluorescent (F)PS-MNPs were detected on the basolateral side, while 1000 nm FPS-MNPs were undetectable. Confocal microscopy showed the uptake of 50 and 200 nm FPS-MNPs by the BeWo b30 and HUVEC cell layer, whereas the 1000 nm FPS-MNPs were only found within the BeWo b30 cell layer. Exposure to PS-MNPs (sizes of 50, 200 and 1000 nm at concentrations of 1–10 µg/mL) did not result in an effect on mitochondrial activity, oxidative stress and gene expression of several functional markers and steroidogenic enzymes. However, LC/MS-MS analysis of the culture media showed a decrease of 17 % in the level of 17-alpha-estradiol after 72-hour exposure to 1 µg/mL 50 nm PS-MNPs compared to vehicle control. Overall, our data showed limited effects of PS-MNPs on placental cell function <em>in vitro</em>, but FPS-MNPs were internalized and detected on the basolateral side in the co-culture. This warrants further studies on effects of MNPs on placental cell function, and particularly steroidogenesis, to assess the potential effects of MNPs during pregnancy.</div></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":"136 ","pages":"Article 108956"},"PeriodicalIF":2.8000,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproductive toxicology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0890623825001273","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"REPRODUCTIVE BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

The placenta plays a crucial role during pregnancy, yet effective in vitro models for placental toxicity testing are limited. In this study, a Transwell co-culture model combining BeWo b30 and HUVEC cells was developed and characterized, and used to study the transport and effects of polystyrene micro- and nanoplastics (PS-MNPs). After 72 h, 8.7 % of 50 nm and 1.2 % of 200 nm of fluorescent (F)PS-MNPs were detected on the basolateral side, while 1000 nm FPS-MNPs were undetectable. Confocal microscopy showed the uptake of 50 and 200 nm FPS-MNPs by the BeWo b30 and HUVEC cell layer, whereas the 1000 nm FPS-MNPs were only found within the BeWo b30 cell layer. Exposure to PS-MNPs (sizes of 50, 200 and 1000 nm at concentrations of 1–10 µg/mL) did not result in an effect on mitochondrial activity, oxidative stress and gene expression of several functional markers and steroidogenic enzymes. However, LC/MS-MS analysis of the culture media showed a decrease of 17 % in the level of 17-alpha-estradiol after 72-hour exposure to 1 µg/mL 50 nm PS-MNPs compared to vehicle control. Overall, our data showed limited effects of PS-MNPs on placental cell function in vitro, but FPS-MNPs were internalized and detected on the basolateral side in the co-culture. This warrants further studies on effects of MNPs on placental cell function, and particularly steroidogenesis, to assess the potential effects of MNPs during pregnancy.
聚苯乙烯微塑料和纳米塑料在生理相关的体外人胎盘共培养模型中的有限传代和功能影响。
胎盘在怀孕期间起着至关重要的作用,但有效的体外胎盘毒性测试模型有限。本研究建立了BeWo b30与HUVEC细胞的Transwell共培养模型,并对其进行了表征,用于研究聚苯乙烯微纳米塑料(PS-MNPs)的转运及其效应。72h后,基底侧检测到8.7%的50nm和1.2%的200nm荧光(F)PS-MNPs,而1000nm的PS-MNPs未检测到。共聚焦显微镜显示,BeWo b30和HUVEC细胞层摄取了50和200nm的FPS-MNPs,而1000nm的FPS-MNPs仅在BeWo b30细胞层中发现。暴露于PS-MNPs(尺寸为50、200和1000nm,浓度为1-10 μ g/mL)对线粒体活性、氧化应激和几种功能标记物和甾体生成酶的基因表达没有影响。然而,LC/MS-MS分析显示,与对照相比,1µg/mL 50nm PS-MNPs暴露72小时后,17- α -雌二醇水平下降了17%。总体而言,我们的数据显示PS-MNPs对体外胎盘细胞功能的影响有限,但在共培养中,PS-MNPs被内化并在基底外侧检测。这就需要进一步研究MNPs对胎盘细胞功能的影响,特别是类固醇生成的影响,以评估MNPs在妊娠期间的潜在影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Reproductive toxicology
Reproductive toxicology 生物-毒理学
CiteScore
6.50
自引率
3.00%
发文量
131
审稿时长
45 days
期刊介绍: Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine. All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信