Comprehensive biomedicine assessment of Apteranthes tuberculata extracts: Phytochemical analysis and multifaceted pharmacological evaluation in animal models.

Abstract

Background: This study comprehensively analyzes the pharmacological effects of ethanolic extract of Apteranthes tuberculata (EEAT) on various physiological parameters in mice.

Objective: The research aimed to quantify flavonoid and phenol contents across different extraction methods, with a focus on the superior efficacy of the ethanolic extract.

Methods: In vitro assays were conducted to assess the antioxidant activity of EEAT, revealing dose-dependent effects and significant inhibition percentages.

Results: EEAT exhibited notable analgesic effects in the writhing response test, particularly at 200 mg/kg, indicating its potential as a natural analgesic. Additionally, its anti-inflammatory effects were complex and dose-dependent in a carrageenan-induced paw edema model. The extract also showed significant changes in body temperature regulation following Brewer's yeast-induced fever, revealing a distinct pattern of initial hypothermia followed by gradual re-elevation. Furthermore, EEAT demonstrated regulatory effects on gastrointestinal motility, with higher doses enhancing intestinal transit in charcoal meal tests.

Conclusion: This study highlights the pharmacological potential of EEAT as a natural therapeutic agent for antioxidant, analgesic, anti-inflammatory, and gastrointestinal regulation, warranting further investigation into its mechanisms of action and therapeutic applications.