The role of gut microbiota-gonadal axis in ovary activation of Asian honey bee (Apis cerana) queens.

IF 9.2 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

npj Biofilms and Microbiomes Pub Date : 2025-06-07 DOI:10.1038/s41522-025-00723-9

Chonghui Zhao, Yehua Peng, Wenfeng Li, Muhammad Fahad Raza, Wenbo Wang, Yi Zhang, Yanping Chen, Jun Guo, Shaokang Huang, Richou Han

{"title":"The role of gut microbiota-gonadal axis in ovary activation of Asian honey bee (Apis cerana) queens.","authors":"Chonghui Zhao, Yehua Peng, Wenfeng Li, Muhammad Fahad Raza, Wenbo Wang, Yi Zhang, Yanping Chen, Jun Guo, Shaokang Huang, Richou Han","doi":"10.1038/s41522-025-00723-9","DOIUrl":null,"url":null,"abstract":"<p><p>The gut microbiota-gonadal axis is increasingly recognized, but its reproductive roles remain unclear. Here, we used the Asian honey bee Apis cerana queens as a model to investigate the role of the gut microbiota-gonadal axis on ovary activation. By artificially caging and releasing the mated queens for a short or long period and monitoring the morphological changes of their ovaries, we confirmed that the activation and suppression of the queen ovary could be switched quickly. We found that the ovary weight was positively correlated with the body weight. 16S rRNA sequencing showed ovarian deactivation reduced gut Lactobacillus abundance. Untargeted metabolomics identified purine metabolism as the dominant ovarian pathway, while correlation analyses implicated Lactobacillus in modulating ovarian morphology through purine signaling. This study elucidates microbiota-gonadal crosstalk governing reproduction, providing mechanistic insights with translational potential for reproductive health management.</p>","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"97"},"PeriodicalIF":9.2000,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145443/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"npj Biofilms and Microbiomes","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1038/s41522-025-00723-9","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The gut microbiota-gonadal axis is increasingly recognized, but its reproductive roles remain unclear. Here, we used the Asian honey bee Apis cerana queens as a model to investigate the role of the gut microbiota-gonadal axis on ovary activation. By artificially caging and releasing the mated queens for a short or long period and monitoring the morphological changes of their ovaries, we confirmed that the activation and suppression of the queen ovary could be switched quickly. We found that the ovary weight was positively correlated with the body weight. 16S rRNA sequencing showed ovarian deactivation reduced gut Lactobacillus abundance. Untargeted metabolomics identified purine metabolism as the dominant ovarian pathway, while correlation analyses implicated Lactobacillus in modulating ovarian morphology through purine signaling. This study elucidates microbiota-gonadal crosstalk governing reproduction, providing mechanistic insights with translational potential for reproductive health management.

查看原文本刊更多论文

肠道微生物-性腺轴在亚洲蜜蜂（Apis cerana）蜂王卵巢激活中的作用。

肠道微生物-性腺轴越来越被认识到，但其生殖作用仍不清楚。本研究以亚洲蜜蜂蜂（Apis cerana queens）为模型，研究肠道微生物-性腺轴对卵巢激活的作用。通过人工圈养或长时间放生交配后的蜂王，观察其卵巢形态变化，证实了蜂王卵巢的激活和抑制可以快速切换。我们发现卵巢重量与体重呈正相关。16S rRNA测序显示卵巢失活降低了肠道乳酸杆菌的丰度。非靶向代谢组学发现嘌呤代谢是卵巢的主要途径，而相关分析表明乳杆菌通过嘌呤信号调节卵巢形态。本研究阐明了微生物-性腺串扰控制生殖，为生殖健康管理提供了具有翻译潜力的机制见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology

CiteScore

12.10

自引率

3.30%

发文量

审稿时长

9 weeks

期刊介绍： npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.