Candida auris clade I isolates from ear discharge in Japan demonstrated comparable virulence to invasive clade I isolates against Galleria mellonella

IF 1.9 4区医学 Q3 INFECTIOUS DISEASES

Journal of Infection and Chemotherapy Pub Date : 2025-06-04 DOI:10.1016/j.jiac.2025.102747

Sayoko Oiki , Masahiro Abe , Sota Sadamoto , Kazuki Amemiya , Takayuki Shinohara , Amato Otani , Ami Koizumi , Takashi Umeyama , Koichi Makimura , Yoshitsugu Miyazaki

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引用次数: 0

Abstract

Introduction

Candida auris is one of the most serious fungal pathogens in the world due to its characteristics of multidrug resistance and causing outbreaks in hospitals. Based on genetic analysis through whole-genome sequencing, C. auris has been classified into four major clades. In Japan, most strains were isolated from noninvasive ear discharge and classified into clade II, whereas clades I, III, and IV are typically detected in patients with invasive candidiasis worldwide. However, three strains, NIIDFCaurisB0004, LSEM 3732, and LSEM 4020, were isolated from ear discharge in Japan and classified into clade I, and their virulence remains unknown. Here, we demonstrate the virulence of these ear discharge-derived clade I strain using the Galleria mellonella infection model.

Methods

Survival analysis, histopathological analysis, and colony count measurements of G. mellonella larvae infected with C. auris were conducted to compare the virulence of ear discharge-derived clade I strains, ear discharge-derived clade II strains, and non-ear discharge-derived clade I strains.

Results

Ear discharge-derived clade I strains exhibited higher mortality rates than ear discharge-derived clade II strains, comparable to those of invasive clade I strains. Histopathological analysis and colony count measurements revealed that the ear discharge-derived clade I strain, NIIDFCaurisB0004, exhibited significantly more growth than clade II strains in G. mellonella.

Conclusion

Even if C. auris is isolated from ear discharge in Japan, there exists a possibility that it can be classified into clade I with high virulence. Therefore, detailed identification of the clade is essential.

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从日本耳部分泌物中分离出的耳念珠菌进化支I与侵入性进化支I对mellonella的毒力相当。

摘要耳念珠菌具有多药耐药和在医院暴发的特点，是世界上最严重的真菌病原体之一。基于全基因组测序的遗传分析，auris被划分为4个主要支系。在日本，大多数菌株是从非侵入性耳部分泌物中分离出来的，并被归类为II支，而在世界范围内，侵袭性念珠菌病患者中通常检测到I、III和IV支。然而，从日本耳部分泌物中分离到3株NIIDFCaurisB0004、LSEM 3732和LSEM 4020，其毒力尚不清楚。在这里，我们展示了这些耳部分泌物衍生分支I菌株的毒力，使用mellonella感染模型。方法：采用生存分析、组织病理学分析和菌落计数等方法，比较耳液源分支I株、耳液源分支II株和非耳液源分支I株的毒力。结果：耳部分泌物衍生的进化支I株的死亡率高于耳部分泌物衍生的进化支II株，与入侵进化支I株的死亡率相当。组织病理学分析和菌落计数结果显示，耳液衍生的I枝菌株NIIDFCaurisB0004在mellonella中表现出明显高于II枝菌株的生长。结论：即使从日本的耳分泌物中分离到耳球菌，也有可能将其归入高毒力的I支。因此，对进化枝的详细鉴定是必不可少的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Infection and Chemotherapy INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY

CiteScore

4.10

自引率

4.50%

发文量

303

审稿时长

47 days

期刊介绍： The Journal of Infection and Chemotherapy (JIC) — official journal of the Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases — welcomes original papers, laboratory or clinical, as well as case reports, notes, committee reports, surveillance and guidelines from all parts of the world on all aspects of chemotherapy, covering the pathogenesis, diagnosis, treatment, and control of infection, including treatment with anticancer drugs. Experimental studies on animal models and pharmacokinetics, and reports on epidemiology and clinical trials are particularly welcome.