Phytochemical studies of Veitchia arecina Becc. leaves with evaluation of in-vitro, in-vivo anti-inflammatory, and wound healing properties in rats.

IF 5.3 2区医学 Q2 IMMUNOLOGY

Inflammopharmacology Pub Date : 2025-06-09 DOI:10.1007/s10787-025-01802-1

Radwa S Emad, Rasha A Attia, Mona Fekry, Nesreen M I M Elkomy, Tarek M Ibrahim, Amal A Al-Gendy

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引用次数: 0

Abstract

Veitchia arecina Becc. (Arecaceae family), a palm species known for its antioxidant and anti-inflammatory properties, attributed to bioactive constituents that may facilitate wound healing and enhance dermatological health. However, the wound-healing efficacy of Veitchia arecina remains uninvestigated. This study is the first to evaluate the chemical composition, antioxidant and anti-inflammatory activities, dermal safety, and wound-healing potential of Veitchia arecina leaf fractions in a rat model. The study aimed to elucidate their mechanism of action in tissue regeneration. The chemical composition of light petroleum ether and n-butanol soluble fractions of Veitchia arecina was analyzed via gas-liquid chromatography-mass spectrometry (GLC-MS) and liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS), respectively. In-vitro antioxidant (DPPH), anti-inflammatory (COX-1), and MTT assays were performed utilizing a 5% gel formulation, with povidone-iodine serving as the standard. The phytochemical analysis of the tested fractions using GLC-MS and LC-ESI-MS/MS revealed the presence of sterols, terpenes, vitamin E isomers, and fatty acids in light petroleum ether soluble fraction as well as flavonoid glycosides and phenolics in n-butanol soluble fraction. Both fractions showed significant antioxidant and COX-1 inhibitory activities comparable to Trolox and ibuprofen. The topical gel formulations exhibited no dermal toxicity while improving healing by attenuating inflammation and oxidative stress, enhancing blood vessel formation, re-epithelization, formation of collagen, and restoring skin architecture. Notably, the n-butanol fraction demonstrated superior efficacy in facilitating granulation tissue maturation. This study demonstrates the dermal safety, antioxidant, anti-inflammatory, and wound-healing properties of Veitchia arecina leaf fractions, particularly flavonoid-rich variants, suggesting their potential therapeutic applications in dermatological conditions.

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槟榔子的植物化学研究。叶片对大鼠体内、体外抗炎和伤口愈合性能的评价。

维契亚是中国的一个城市。（槟榔科），一种以其抗氧化和抗炎特性而闻名的棕榈物种，归因于可能促进伤口愈合和增进皮肤健康的生物活性成分。然而，槟榔的创面愈合效果尚未得到研究。本研究首次在大鼠模型上对槟榔叶的化学成分、抗氧化和抗炎活性、皮肤安全性和伤口愈合潜力进行了评价。本研究旨在阐明它们在组织再生中的作用机制。采用气相色谱-质谱联用技术（GLC-MS）和液相色谱-电喷雾串联质谱联用技术（LC-ESI-MS/MS）分析了槟槟酒中轻质石油醚和正丁醇可溶性组分的化学成分。体外抗氧化（DPPH）、抗炎（COX-1）和MTT测定采用5%凝胶配方，聚维酮碘作为标准。采用GLC-MS和LC-ESI-MS/MS对所测组分进行植物化学分析，发现轻石油醚可溶性组分中含有甾醇类、萜烯类、维生素E异构体和脂肪酸，正丁醇可溶性组分中含有类黄酮苷类和酚类物质。两组分均表现出与Trolox和ibuprofen相当的抗氧化和COX-1抑制活性。局部凝胶配方没有皮肤毒性，同时通过减轻炎症和氧化应激、促进血管形成、再上皮化、胶原蛋白形成和恢复皮肤结构来促进愈合。值得注意的是，正丁醇部分在促进肉芽组织成熟方面表现出优越的功效。该研究证明了槟榔叶的皮肤安全性、抗氧化、抗炎和伤口愈合特性，特别是富含类黄酮的变体，表明其在皮肤病中的潜在治疗应用。

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来源期刊

Inflammopharmacology IMMUNOLOGYTOXICOLOGY-TOXICOLOGY

CiteScore

8.00

自引率

3.40%

发文量

200

期刊介绍： Inflammopharmacology is the official publication of the Gastrointestinal Section of the International Union of Basic and Clinical Pharmacology (IUPHAR) and the Hungarian Experimental and Clinical Pharmacology Society (HECPS). Inflammopharmacology publishes papers on all aspects of inflammation and its pharmacological control emphasizing comparisons of (a) different inflammatory states, and (b) the actions, therapeutic efficacy and safety of drugs employed in the treatment of inflammatory conditions. The comparative aspects of the types of inflammatory conditions include gastrointestinal disease (e.g. ulcerative colitis, Crohn''s disease), parasitic diseases, toxicological manifestations of the effects of drugs and environmental agents, arthritic conditions, and inflammatory effects of injury or aging on skeletal muscle. The journal has seven main interest areas: -Drug-Disease Interactions - Conditional Pharmacology - i.e. where the condition (disease or stress state) influences the therapeutic response and side (adverse) effects from anti-inflammatory drugs. Mechanisms of drug-disease and drug disease interactions and the role of different stress states -Rheumatology - particular emphasis on methods of measurement of clinical response effects of new agents, adverse effects from anti-rheumatic drugs -Gastroenterology - with particular emphasis on animal and human models, mechanisms of mucosal inflammation and ulceration and effects of novel and established anti-ulcer, anti-inflammatory agents, or antiparasitic agents -Neuro-Inflammation and Pain - model systems, pharmacology of new analgesic agents and mechanisms of neuro-inflammation and pain -Novel drugs, natural products and nutraceuticals - and their effects on inflammatory processes, especially where there are indications of novel modes action compared with conventional drugs e.g. NSAIDs -Muscle-immune interactions during inflammation [...]