Progestogen is a potential trigger initiating sex inversion in rice field eel (Monopterus albus).

IF 2.5 3区农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Fish Physiology and Biochemistry Pub Date : 2025-06-09 DOI:10.1007/s10695-025-01524-9

Xiancheng Qu, Jiaoyun Jiang, Min Li, Jianhua Ye, Lisa Pan, Qinhua Zhang, Guofang Zhong, Xinchao Zhu, Yifang Du, Xianhui Li

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Abstract

The rice field eel or swamp eel (Monopterus albus) is a hermaphroditic freshwater teleost fish that transitions from a functional female to a functional male. However, the biological mechanism of sex inversion in this species remains unclear. Here we clone the 5' proximal flanking region of the rice field eel dmrt1 gene and find that the progesterone response element (PRE) and androgen response element (ARE) uniquely exist in this region. More importantly, based on reporter gene analysis, in vitro tissue culture, and progestogen hormone level assays, we reveal that progestogen is the prerequisite for the sex inversion of the rice field eel, and androgen plays an important role in the positive regulation of the sex inversion process. Collectively, our results suggest that progestogen is the trigger that initiates the rice field eel's sex trans-differentiation during final oocyte maturation and clarifies the biological mechanism of sex inversion in the rice field eel.

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黄鳝（Monopterus albus）的孕激素是引发性反转的潜在因素。

稻田鳗鱼或沼泽鳗鱼（黄鳝）是一种雌雄同体的淡水硬骨鱼，从功能性的雌性转变为功能性的雄性。然而，该物种性别反转的生物学机制尚不清楚。本研究克隆了稻田鳗dmrt1基因近侧5'区域，发现该区域中只存在孕激素应答元件（PRE）和雄激素应答元件（ARE）。更重要的是，通过报告基因分析、体外组织培养和孕激素水平检测，我们发现孕激素是稻田鳗性别反转的先决条件，雄激素在性别反转过程中起着重要的正向调节作用。综上所述，我们的研究结果表明，孕激素是在最终卵母细胞成熟过程中启动稻田鳗鱼性别反分化的触发因素，并阐明了稻田鳗鱼性别反转的生物学机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fish Physiology and Biochemistry 农林科学-生化与分子生物学

CiteScore

5.60

自引率

6.90%

发文量

106

审稿时长

4 months

期刊介绍： Fish Physiology and Biochemistry is an international journal publishing original research papers in all aspects of the physiology and biochemistry of fishes. Coverage includes experimental work in such topics as biochemistry of organisms, organs, tissues and cells; structure of organs, tissues, cells and organelles related to their function; nutritional, osmotic, ionic, respiratory and excretory homeostasis; nerve and muscle physiology; endocrinology; reproductive physiology; energetics; biochemical and physiological effects of toxicants; molecular biology and biotechnology and more.