Jingfang Granules for Diabetic Wound Healing: Insights from Network Pharmacology and Experimental Validation.

IF 4.7 2区医学 Q1 CHEMISTRY, MEDICINAL

Drug Design, Development and Therapy Pub Date : 2025-06-04 eCollection Date: 2025-01-01 DOI:10.2147/DDDT.S516298

Rongrong Wang, Qian Wang, Mingfei Liu, He Xiao, Guimin Zhang, Jingchun Yao, Ming Liu

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引用次数: 0

Abstract

Background: Diabetic wounds are one of the most common complications of diabetes mellitus. Jingfang Granules (JFG), a combination of 11 herbs, has been clinically used for treating colds and the flu and for preventing various skin diseases.

Purpose: The present study was designed to evaluate the therapeutic effect of JFG on diabetic wounds and to elucidate the associated mechanisms.

Methods: JFG serum was prepared using Sprague-Dawley rats and the phytochemicals of JFG in the serum were identified using UHPLC-ESI-QE-Orbitrap-MS. A cell viability assay and cellular angiogenesis methods were performed to evaluate wound healing in vitro. Diabetic wounds were developed using streptozotocin-induced diabetic rats to investigate the efficacy of JFG on diabetic wounds in vivo. Network pharmacology analysis, molecular docking, and Western blot were performed to elucidate the potential mechanisms of JFG in diabetic wound healing.

Results: JFG serum attenuated H₂O₂-induced and high glucose-induced oxidative damage, significantly reduced lipopolysaccharide-induced upregulation of inflammatory cytokines, and promoted angiogenesis in vitro. In diabetic rats, JFG effectively promoted wound healing, reduced blood glucose and lipid levels, and alleviated oxidative stress and inflammation. A total of 56 phytochemicals were identified in the JFG serum. Six core targets (AKT1, EGFR, MAPK3, MAPK1, IL6, and TNF) and the PI3K-AKT and MAPK signaling pathways were identified by network pharmacology analysis, which were further validated by subsequent experimental methods.

Conclusion: JFG could accelerate diabetic wound healing by alleviating oxidative damage, suppressing inflammation, promoting angiogenesis, and regulating metabolic abnormalities, with involvement of the PI3K-AKT and MAPK signaling pathways.

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精方颗粒对糖尿病创面愈合的网络药理学见解及实验验证。

背景：糖尿病伤口是糖尿病最常见的并发症之一。精方颗粒（JFG）由11种草药组成，临床上用于治疗感冒和流感，并预防各种皮肤病。目的：评价JFG对糖尿病创面的治疗作用，并探讨其作用机制。方法：采用Sprague-Dawley大鼠制备JFG血清，采用UHPLC-ESI-QE-Orbitrap-MS对血清中JFG的植物化学成分进行鉴定。采用细胞活力测定和细胞血管生成方法评价体外创面愈合。以链脲佐菌素诱导的糖尿病大鼠为实验动物，观察JFG对糖尿病创面的治疗作用。通过网络药理学分析、分子对接、Western blot等方法探讨JFG在糖尿病创面愈合中的作用机制。结果：JFG血清减轻h2o2诱导和高糖诱导的氧化损伤，显著降低脂多糖诱导的炎性细胞因子上调，促进体外血管生成。在糖尿病大鼠中，JFG能有效促进创面愈合，降低血糖和血脂水平，减轻氧化应激和炎症。在JFG血清中鉴定出56种植物化学物质。通过网络药理学分析确定6个核心靶点（AKT1、EGFR、MAPK3、MAPK1、IL6和TNF）和PI3K-AKT和MAPK信号通路，并通过后续实验方法进一步验证。结论：JFG可通过减轻氧化损伤、抑制炎症、促进血管生成、调节代谢异常等途径促进糖尿病创面愈合，其机制与PI3K-AKT和MAPK信号通路有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Drug Design, Development and Therapy CHEMISTRY, MEDICINAL-PHARMACOLOGY & PHARMACY

CiteScore

9.00

自引率

0.00%

发文量

382

审稿时长

>12 weeks

期刊介绍： Drug Design, Development and Therapy is an international, peer-reviewed, open access journal that spans the spectrum of drug design, discovery and development through to clinical applications. The journal is characterized by the rapid reporting of high-quality original research, reviews, expert opinions, commentary and clinical studies in all therapeutic areas. Specific topics covered by the journal include: Drug target identification and validation Phenotypic screening and target deconvolution Biochemical analyses of drug targets and their pathways New methods or relevant applications in molecular/drug design and computer-aided drug discovery* Design, synthesis, and biological evaluation of novel biologically active compounds (including diagnostics or chemical probes) Structural or molecular biological studies elucidating molecular recognition processes Fragment-based drug discovery Pharmaceutical/red biotechnology Isolation, structural characterization, (bio)synthesis, bioengineering and pharmacological evaluation of natural products** Distribution, pharmacokinetics and metabolic transformations of drugs or biologically active compounds in drug development Drug delivery and formulation (design and characterization of dosage forms, release mechanisms and in vivo testing) Preclinical development studies Translational animal models Mechanisms of action and signalling pathways Toxicology Gene therapy, cell therapy and immunotherapy Personalized medicine and pharmacogenomics Clinical drug evaluation Patient safety and sustained use of medicines.