Gas Chromatography-Mass Spectrometry Analysis, Genoprotective, and Antioxidant Potential of Curio Radicans (L. f.) P.V. Heath.

IF 2.5 4区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY
Muhammad Naseer, Muhammad Adil, Sarir Ahmad, Mikhlid H Almutari, Abdulwahed Fahad Alrefaei, Sajid Ali, Fayyaz Asad
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Abstract

Natural substances play a crucial role in modern medicine by targeting cellular pathways associated with diseases. Medicinal plants, rich in bioactive compounds, offer promising therapeutic potential. This study examines the phytochemicals and biological activity of Curio radicans extracts. Gas chromatography-mass spectrometry analysis identifies 29 compounds in the ethanolic extract and 7 in the ethyl acetate extract. The ethanolic extract contains 3,4-Bis (Methoxycarbonyl)furan (15.76%) and 4-Imidazolidinone (14.17%), while the ethyl acetate extract is dominated by Diisooctyl phthalate (49.25%). Genoprotective activity, using the comet assay on human lymphocytes, demonstrates significant dose-dependent reductions in H2O2-induced DNA damage. The ethyl acetate extract reduces total comet score (TCS) from 265.3 ± 20.4 to 33.0 ± 4.3 at 100 mg/100 mL (p < 0.0001), outperforming the ethanolic extract, which reduced TCS from 226.7 ± 41.6 to 44.0 ± 7.2 (p < 0.001). Antioxidant activity, assessed via the DPPH method, reveals moderate activity for the ethyl acetate extract (72.61 ± 1.65%, IC50: 137.58 μg/ml) and lower activity for the ethanolic extract (67.52 ± 0.44%, IC50: 156.52 μg/ml). These findings underscore the therapeutic potential of C. radicans, particularly the ethyl acetate extract, which demonstrates genoprotective effects. Researchers should focus on isolating active compounds and exploring their potential in drug development.

Curio Radicans的气相色谱-质谱分析、基因保护和抗氧化潜力(L. f.)pv健康。
天然物质通过靶向与疾病相关的细胞通路,在现代医学中发挥着至关重要的作用。药用植物富含生物活性化合物,具有良好的治疗潜力。本研究探讨了古里根提取物的植物化学成分和生物活性。气相色谱-质谱分析鉴定乙醇提取物中有29种化合物,乙酸乙酯提取物中有7种化合物。乙醇提取物中含有3,4-双(甲氧羰基)呋喃(15.76%)和4-咪唑烷酮(14.17%),乙酸乙酯提取物中以邻苯二甲酸二异辛酯为主(49.25%)。使用彗星测定人类淋巴细胞的基因保护活性表明,h2o2诱导的DNA损伤显着的剂量依赖性降低。乙酸乙酯提取物在100 mg/100 mL时,总彗星评分(TCS)从265.3±20.4降至33.0±4.3 (p 50: 137.58 μg/ mL),而乙醇提取物的活性降低(67.52±0.44%,IC50: 156.52 μg/ mL)。这些发现强调了根状芽孢杆菌的治疗潜力,特别是乙酸乙酯提取物,它显示了基因保护作用。研究人员应将重点放在分离活性化合物和探索其在药物开发中的潜力上。
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来源期刊
ChemistryOpen
ChemistryOpen CHEMISTRY, MULTIDISCIPLINARY-
CiteScore
4.80
自引率
4.30%
发文量
143
审稿时长
1 months
期刊介绍: ChemistryOpen is a multidisciplinary, gold-road open-access, international forum for the publication of outstanding Reviews, Full Papers, and Communications from all areas of chemistry and related fields. It is co-owned by 16 continental European Chemical Societies, who have banded together in the alliance called ChemPubSoc Europe for the purpose of publishing high-quality journals in the field of chemistry and its border disciplines. As some of the governments of the countries represented in ChemPubSoc Europe have strongly recommended that the research conducted with their funding is freely accessible for all readers (Open Access), ChemPubSoc Europe was concerned that no journal for which the ethical standards were monitored by a chemical society was available for such papers. ChemistryOpen fills this gap.
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