Immunophenotypic Profiling Reveals Circulating Lymphocyte Dysregulation in Primary Sjögren's Syndrome

IF 2.4 4区医学 Q2 RHEUMATOLOGY

International Journal of Rheumatic Diseases Pub Date : 2025-06-09 DOI:10.1111/1756-185X.70276

Xianghui Wen, Yanli Zhang, Xinyu Wu, Xuqi Zheng, Mingcan Yang, Dong Liu, Budian Liu, Qiujing Wei, Xiehui Chen, Peng Zhang, Jieruo Gu

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引用次数: 0

Abstract

Objectives

To investigate peripheral T and B cell subset alterations in primary Sjögren's syndrome (pSS) and their associations with disease activity (ESSDAI).

Methods

This study enrolled 43 patients with pSS and 46 healthy controls (HCs). Demographic data and clinical manifestations were collected for all participants. Flow cytometry was utilized to detect the frequencies of T and B cell subsets in peripheral blood mononuclear cells.

Results

Primary Sjögren's syndrome patients exhibited elevated naïve CD4+ T cells, central memory CD4+ T cells, virus-specific CD8+ T cells, Th2/Th17 cells, total B cells, and CD21−/low B cells. Conversely, terminally differentiated/exhausted CD4+ T cells, Th1/Tc1 cells, and plasma cells were reduced. ESSDAI correlated positively with Tc cells and CD21−/low B cells and inversely with plasma cells.

Conclusions

T and B cell dysregulation may drive immune dysfunction in pSS, with specific subsets linked to disease severity.

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免疫表型分析揭示原发性Sjögren综合征的循环淋巴细胞失调

目的探讨原发性Sjögren综合征（pSS）外周血T和B细胞亚群的改变及其与疾病活动性（ESSDAI）的关系。方法本研究纳入43例pSS患者和46例健康对照。收集所有参与者的人口学资料和临床表现。流式细胞术检测外周血单个核细胞中T细胞亚群和B细胞亚群的频率。结果原发性Sjögren综合征患者表现出naïve CD4+ T细胞、中枢记忆CD4+ T细胞、病毒特异性CD8+ T细胞、Th2/Th17细胞、总B细胞和CD21−/低B细胞的升高。相反，终末分化/耗竭的CD4+ T细胞、Th1/Tc1细胞和浆细胞减少。ESSDAI与Tc细胞和CD21−/低B细胞呈正相关，与浆细胞呈负相关。结论：T细胞和B细胞失调可能驱动pSS患者的免疫功能障碍，其特异性亚群与疾病严重程度相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Rheumatic Diseases RHEUMATOLOGY-

CiteScore

3.70

自引率

4.00%

发文量

362

审稿时长

1 months

期刊介绍： The International Journal of Rheumatic Diseases (formerly APLAR Journal of Rheumatology) is the official journal of the Asia Pacific League of Associations for Rheumatology. The Journal accepts original articles on clinical or experimental research pertinent to the rheumatic diseases, work on connective tissue diseases and other immune and allergic disorders. The acceptance criteria for all papers are the quality and originality of the research and its significance to our readership. Except where otherwise stated, manuscripts are peer reviewed by two anonymous reviewers and the Editor.