Changes in Serum Proteomic Profiles at Different Stages of Pregnancy Toxemia in Goats

IF 2.2 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine Pub Date : 2025-06-10 DOI:10.1111/jvim.70139

Murat Uztimür, Cennet Nur Ünal, Gürler Akpınar

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引用次数: 0

Abstract

Background

In human medicine, serum proteome profiles are used to differentiate metabolic diseases and evaluate their pathophysiology. Understanding the serum proteome profiles of goats with pregnancy toxemia might help identify the proteomes and pathways responsible for the development of this disease and improve diagnosis and treatment.

Hypothesis/Aim

Determine biomarkers that differentiate healthy goats from goats with subclinical pregnancy toxemia (SPT) and clinical pregnancy toxemia (CPT) using serum proteome analysis. In addition, serum protein differences were evaluated to distinguish between SPT and CPT.

Animals

Thirty-five hair goats were included, with 15 in the SPT group, 10 in the CPT group, and 10 in the control group.

Methods

The cross-sectional study was conducted with animals from goat farms in Bingol, Türkiye, between December 2023 and May 2024. Serum samples were investigated using mass spectrometry-based proteomic analysis.

Results

Proteomic profiles showed significant variation in relative protein abundance. Twenty-five proteins were significantly (p ≤ 0.01) differentially expressed between animals with pregnancy toxemia and those of the control group, with ≥ 2-fold changes in abundance. Proteins involved in cellular, biological, and molecular processes, including processes related to reactive oxygen species, cytokine activation, acute phase response signaling, lipid metabolism, and antimicrobial activity were altered in animals with pregnancy toxemia.

Conclusion and Clinical Importance

The proteomic candidates identified are biomarkers that may facilitate the diagnosis, treatment, and management of pregnancy toxemia in goats. Serum proteomic biomarkers could be used in rapid tests in the future and may improve the management of pregnancy toxemia on farms.

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山羊妊娠毒血症不同阶段血清蛋白质组学特征的变化

在人类医学中，血清蛋白质组谱被用来区分代谢性疾病和评估其病理生理。了解妊娠毒血症山羊的血清蛋白质组谱可能有助于确定导致该疾病发展的蛋白质组和途径，并改善诊断和治疗。假设/目的通过血清蛋白质组分析确定区分健康山羊与亚临床妊娠毒血症（SPT）和临床妊娠毒血症（CPT）的生物标志物。此外，评估血清蛋白差异以区分SPT和CPT。试验选用毛山羊35只，其中SPT组15只，CPT组10只，对照组10只。方法对2023年12月至2024年5月期间来自基耶省宾戈尔山羊农场的动物进行横断面研究。血清样本采用基于质谱的蛋白质组学分析进行研究。结果蛋白质组学分析显示，不同人群的相对蛋白丰度存在显著差异。妊娠毒血症组与对照组有25个蛋白表达差异显著（p≤0.01），丰度变化≥2倍。参与细胞、生物和分子过程的蛋白质，包括与活性氧、细胞因子激活、急性期反应信号、脂质代谢和抗菌活性相关的过程，在妊娠毒血症动物中发生了改变。结论及其临床意义鉴定出的候选蛋白质组学标记物可能有助于山羊妊娠毒血症的诊断、治疗和管理。血清蛋白质组学生物标志物可用于未来的快速检测，并可能改善农场妊娠毒血症的管理。

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来源期刊

Journal of Veterinary Internal Medicine 农林科学-兽医学

CiteScore

4.50

自引率

11.50%

发文量

243

审稿时长

22 weeks

期刊介绍： The mission of the Journal of Veterinary Internal Medicine is to advance veterinary medical knowledge and improve the lives of animals by publication of authoritative scientific articles of animal diseases.