Nano-apatite with Doxycycline for pulp capping: A potential strategy to reduce inflammation and promote pulp healing

Abstract

The aim of the study is to develop a specialist dental capping agent that is particularly formulated to target pulp inflammation. The study included the synthesis of nano-apatite using Lepidium sativum (nHALS) that was loaded with Doxycycline (nHALS@DXC) utilizing an in vitro model of pulpitis with an O-antigen (O-A). The nanoscale HALS were analysed using scanning electron microscopy (SEM) and Transmission electron microscopy (TEM) to assess their odontogenic properties. The diameter of the nHALS nanoparticles ranged from 20 to 33 nm in width and 80–170 nm in length. Doxycycline has been identified as an appropriate loading material for medicinal applications. While the effect of nHALS@DXC on the odontogenic competence of HDPSCs was not superior to that of nHALS alone, it exhibited a more significant anti-inflammatory effect. The findings indicate that nHALS@DXC has the capacity to serve as an effective agent for pulp capping, perhaps surpassing nHALS in terms of reducing inflammation and activating dental pup cells to enhance pulp repair after injury.