A disposable VHH-based SPR fiber probe for sensitive and specific detection of MMP-9 in cancer and inflammatory disease

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-06-09 DOI:10.1016/j.bios.2025.117684

Dandan Wu , Jian Yang , Junxiao Cong , Jian Zhou , Youbin Li , Feng Yan , Xuejin Li , Yuzhi Chen , Liang Ye

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引用次数: 0

Abstract

A novel one-step label-free immunoassay platform was developed through the integration of nanobody (VHH) technology with a disposable surface plasmon resonance (SPR) fiber probe for rapid, precise biomarker detection. The platform employs immobilization of high-affinity VHH against human matrix metalloproteinase-9 (MMP-9), a key biomarker in cancer and inflammatory bowel disease (IBD), on gold-coated optical fiber surfaces. This disposable VHH-based SPR fiber probe enables sensitive MMP-9 quantification in various cancer cell lines as well as tissue and serum specimens from murine models of colorectal and breast cancer and dextran sulfate sodium (DSS)-induced colitis. Clinical validation using human samples demonstrated successful MMP-9 detection in breast cancer and IBD cohorts. The VHH-based SPR fiber probe demonstrated rapid, sensitive, and specific MMP-9 detection with minimal cross-reactivity, establishing its reliability for clinical applications in early cancer and IBD diagnosis, therapeutic monitoring, and prognostic assessment.

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一种基于vhh的一次性SPR纤维探针，用于敏感和特异性检测癌症和炎症性疾病中的MMP-9

通过纳米体（VHH）技术与一次性表面等离子体共振（SPR）纤维探针的集成，开发了一种新的一步无标记免疫分析平台，用于快速，精确的生物标志物检测。该平台将高亲和力的VHH固定在镀金光纤表面，以对抗人类基质金属蛋白酶-9 (MMP-9)， MMP-9是癌症和炎症性肠病（IBD）的关键生物标志物。这种基于vhh的一次性SPR纤维探针能够在各种癌细胞系以及结直肠癌和乳腺癌小鼠模型和葡聚糖硫酸钠（DSS）诱导结肠炎的组织和血清标本中进行敏感的MMP-9定量。使用人类样本的临床验证表明，在乳腺癌和IBD队列中成功检测到MMP-9。基于vhh的SPR纤维探针显示出快速、敏感和特异性的MMP-9检测，交叉反应性最小，在早期癌症和IBD诊断、治疗监测和预后评估方面建立了可靠的临床应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.