Establishment of a microsphere-based flow cytometric assay for quantitative detection of total immunoglobulin E
IF 3.2
3区 医学
Q2 MEDICAL LABORATORY TECHNOLOGY
引用次数: 0
Abstract
Background
In the diagnosis of allergic diseases, in vitro serum IgE level tests were conventionally used. In recent years, microsphere-based flow cytometric assays for IgE have gained more attention. The present study aimed to establish a microsphere-based flow cytometric assay for quantitative detection of tIgE.
Methods
The assay was established after selection of microsphere, antibody pair, detection mode, and optimization of coating ratio. In order to quantitate tIgE, the calibration curve was established. The detection performance of this new tIgE flow cytometric assay was also confirmed. In addition, the correlations between this assay with the ImmunoCAP assay or the immunoturbidimetry assay were evaluated.
Results
This new tIgE flow cytometric assay has low CVs of repeatability (7.62 % and 6.98 %) and intermediate precision (7.50 % and 8.41 %). The detection range was 2 ∼ 6000 IU/mL. The range of linearity was from 2 ∼ 3363 IU/L (r = 0.9932). The correlation coefficient (r) for the correlation analysis between the results of the newly developed tIgE flow cytometric assay and ImmunoCAP assay or immunoturbidimetry assay was 0.9345 and 0.9348, respectively.
Conclusion
A tIgE flow cytometric assay was successfully established. It could be used in clinical laboratories and quantitate lots of allergen-sIgE detected by flow cytometric assay.
建立微球流式细胞术定量检测总免疫球蛋白E的方法
背景在变态反应性疾病的诊断中，常规采用体外血清IgE水平检测。近年来，基于微球的流式细胞术检测IgE得到了越来越多的关注。本研究旨在建立一种基于微球的流式细胞术定量检测tIgE的方法。方法选择微球、抗体对、检测方式、优化包被比，建立检测方法。为了定量测定tIgE，建立了tIgE的标定曲线。这种新的tIgE流式细胞分析方法的检测性能也得到了证实。此外，还评估了该检测与免疫cap检测或免疫比浊法检测之间的相关性。结果该方法重复性CVs值低（7.62%和6.98%），精密度中等（7.50%和8.41%）。检测范围为2 ~ 6000 IU/mL。线性范围为2 ~ 3363 IU/L （r = 0.9932）。新建立的tIgE流式细胞分析结果与ImmunoCAP、免疫比浊法的相关系数r分别为0.9345、0.9348。结论成功建立了tIgE流式细胞术检测方法。该方法可用于临床实验室，对流式细胞术检测到的大量过敏原sige进行定量分析。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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来源期刊
Clinica Chimica Acta
医学-医学实验技术
CiteScore
10.10
自引率
2.00%
发文量
1268
审稿时长
23 days
期刊介绍：
The Official Journal of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC)
Clinica Chimica Acta is a high-quality journal which publishes original Research Communications in the field of clinical chemistry and laboratory medicine, defined as the diagnostic application of chemistry, biochemistry, immunochemistry, biochemical aspects of hematology, toxicology, and molecular biology to the study of human disease in body fluids and cells.
The objective of the journal is to publish novel information leading to a better understanding of biological mechanisms of human diseases, their prevention, diagnosis, and patient management. Reports of an applied clinical character are also welcome. Papers concerned with normal metabolic processes or with constituents of normal cells or body fluids, such as reports of experimental or clinical studies in animals, are only considered when they are clearly and directly relevant to human disease. Evaluation of commercial products have a low priority for publication, unless they are novel or represent a technological breakthrough. Studies dealing with effects of drugs and natural products and studies dealing with the redox status in various diseases are not within the journal''s scope. Development and evaluation of novel analytical methodologies where applicable to diagnostic clinical chemistry and laboratory medicine, including point-of-care testing, and topics on laboratory management and informatics will also be considered. Studies focused on emerging diagnostic technologies and (big) data analysis procedures including digitalization, mobile Health, and artificial Intelligence applied to Laboratory Medicine are also of interest.
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