Frequency of the AC-2 pattern's new variant (AC-30) and detection of different immunological relationships

IF 3.8 3区医学 Q2 IMMUNOLOGY

Clinical immunology Pub Date : 2025-06-05 DOI:10.1016/j.clim.2025.110536

Mehmet Akif Durmuş , Selda Kömeç

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引用次数: 0

Abstract

Aim

This study investigates the frequency and autoantibody profile of the newly defined AC-30 pattern, a variant of the AC-2 dense fine speckled pattern, in patients undergoing antinuclear antibodies (ANA) testing via IIF on HEp-2 cells.

Result

A total of 12,369 samples collected between July 2024 and March 2025 were retrospectively analyzed. Upon re-evaluation, 147 samples (8.78 %) were identified as AC-30. The most common patterns were speckled (29.25 %), dense fine speckled (25.25 %), homogeneous (10.99 %), and AC-30 (8.78 %). Extractable nuclear antigen (ENA) analysis showed that 68.7 % of AC-30 samples were negative, while 31.3 % were positive most frequently for anti-DFS70, anti-dsDNA, anti-PM/Scl, and anti-SSA/Ro60.

Conclusion

Accurate identification of the AC-30 pattern is essential to prevent misclassification as AC-1 or AC-2, which may lead to diagnostic errors. Further multicenter studies are needed to clarify the clinical relevance and diagnostic value of the AC-30 pattern.

查看原文本刊更多论文

AC-2型新变异（AC-30）的频率及不同免疫学关系的检测

目的研究在HEp-2细胞上进行抗核抗体（ANA）检测的患者中，新定义的AC-30型（AC-2致密细斑型的一种变体）的频率和自身抗体谱。结果对2024年7月至2025年3月采集的12369份样本进行回顾性分析。经重新鉴定，147份（8.78%）样品为AC-30。最常见的是斑点型（29.25%）、密集细斑型（25.25%）、均匀型（10.99%）和AC-30型（8.78%）。可提取核抗原（ENA）分析结果显示，68.7%的AC-30样品呈阴性，其中抗dfs70、抗dsdna、抗pm /Scl和抗ssa /Ro60阳性的比例最高，为31.3%。结论准确识别AC-30型是防止误诊为AC-1或AC-2的关键。需要进一步的多中心研究来阐明AC-30模式的临床相关性和诊断价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical immunology 医学-免疫学

CiteScore

12.30

自引率

1.20%

发文量

212

审稿时长

34 days

期刊介绍： Clinical Immunology publishes original research delving into the molecular and cellular foundations of immunological diseases. Additionally, the journal includes reviews covering timely subjects in basic immunology, along with case reports and letters to the editor.