Inoculating domesticated natural bacterial improved short-chain carboxylic acids yield: Focusing on solid content and bacterial consortia

IF 5.6 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products Pub Date : 2025-06-09 DOI:10.1016/j.indcrop.2025.121330

Miaomiao Zhang , Kaibo Liu , Linying Zhu , Jie Liu , Shuzhen Zhou , Yafan Cai , Chun Chang , Xiaoling Zhao

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Abstract

To enhance the conversion efficiency and operational stability of the biomass refining process, this study investigated the effects of two domesticated bacterial consortia on the degradation of rice straw (RS) and the production of short-chain carboxylic acids (SCCAs) were investigated and further analyzed the key genes involved in biomass conversion. The results indicate that inoculating both the domesticated straw-degrading microorganisms (SDM) and the acid-producing microorganisms (APM) into the straw fermentation system with a total solids (TS) content of 5 wt% resulted in the maximum yield of SCCAs on the 6th day, reaching 186.46 mg/g TS. The key genes change found that an increase in the abundance of key genes such as pta (phosphotransacetylase), ack (acetate kinase) and ldh (lactate dehydrogenase) contributed to the efficient biomass conversion of the consortium. In addition, the high abundance of Enterococcaceae (92.70 %) and Lactobacillaceae (97.90 %) contributed to better RS degradation and acid production during anaerobic fermentation (AF). This study indicated that SDM and APM could improve performance and achieve higher SCCAs during AF.

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接种驯化的天然细菌提高短链羧酸产量：重点研究固含量和菌群

为了提高生物质精制过程的转化效率和运行稳定性，本研究考察了两个驯化菌群对水稻秸秆（RS）降解和短链羧酸（SCCAs）生成的影响，并进一步分析了生物质转化的关键基因。结果表明，在总固体物（TS）含量为5 wt%的秸秆发酵体系中接种驯化的秸秆降解微生物（SDM）和产酸微生物（APM），第6天SCCAs产量达到186.46 mg/g TS，关键基因的变化发现，关键基因如pta （phosphotransacetylase）、Ack（醋酸激酶）和LDH（乳酸脱氢酶）有助于该财团的高效生物质转化。此外，高丰度的肠球菌科（92.70 %）和乳酸杆菌科（97.90 %）有助于在厌氧发酵（AF）过程中更好地降解RS和产酸。本研究表明，SDM和APM可以改善AF期间的性能并获得更高的SCCAs。

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来源期刊

Industrial Crops and Products 农林科学-农业工程

CiteScore

9.50

自引率

8.50%

发文量

1518

审稿时长

43 days

期刊介绍： Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.