Assessment of Al18F-NOTA-FAPI-04 PET/CT imaging for visualization and localization of ovarian and peritoneal endometriotic lesions in a rat model

IF 1.7 4区综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES

Journal of Radiation Research and Applied Sciences Pub Date : 2025-06-10 DOI:10.1016/j.jrras.2025.101679

Lijun Yin , Qianhe Xu , Ahmad Alhaskawi , Yongyi Yang , Jie Shi , Xinhui Zhou , Shi Feng , Yinying Zou , Guoliang Xie , Weihua He , Xinhui Su , Zhenfeng Liu , Hui Lu

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Abstract

Endometriosis, a prevalent chronic inflammatory gynecological disorder, is characterized by the ectopic implantation of functional endometrial tissue outside the uterine cavity and is increasingly recognized as a fibrotic condition. The fibroblast activation protein inhibitor ([¹⁸F] FAPI) represents a novel radiotracer targeting fibroblast activation protein (FAP), demonstrating significant promise in imaging both benign and malignant pathologies. This study aimed to investigate the efficacy of [¹⁸F] FAPI as a non-invasive tracer for detecting ovarian and peritoneal endometriosis.

An endometriosis model was established in Sprague-Dawley rats via autologous transplantation of uterine endometrial tissue to the contralateral ovary and abdominal wall. Small-animal PET/CT imaging was performed 60 min post-injection of Al¹⁸F-NOTA-FAPI-04. Quantitative analysis revealed significantly higher tracer uptake in ovarian endometriotic lesions (OELs; SUV_max = 2.53 ± 0.61) compared to adjacent non-lesional ovarian tissue (sOEL; 1.28 ± 0.46, P = 0.031) and contralateral ovarian tissue (CO; 1.15 ± 0.49, P = 0.016). Similarly, peritoneal endometriotic lesions (PELs) exhibited elevated uptake (SUV_max = 1.74 ± 0.15) relative to surrounding peritoneal endometriotic lesions (sPEL; 0.97 ± 0.13, P = 0.001) and abdominal wall muscle (AWM; 0.83 ± 0.12, P = 0.0004). Histopathological and immunohistochemical analyses further validated the imaging findings.

These results demonstrate that Al¹⁸F-NOTA-FAPI-04 PET/CT can effectively differentiate endometriotic lesions from normal tissues, highlighting its potential as a non-invasive diagnostic tool for endometriosis.

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Al18F-NOTA-FAPI-04 PET/CT成像对大鼠卵巢和腹膜子宫内膜异位症病变可视化和定位的评估

子宫内膜异位症是一种常见的慢性炎症性妇科疾病，其特征是功能性子宫内膜组织在子宫腔外异位着床，越来越多地被认为是一种纤维化疾病。成纤维细胞活化蛋白抑制剂（[18F] FAPI）是一种新型的靶向成纤维细胞活化蛋白（FAP）的放射性示踪剂，在良恶性病理成像中都显示出重要的前景。本研究旨在探讨[18F] FAPI作为无创示踪剂检测卵巢和腹膜子宫内膜异位症的疗效。通过自体子宫内膜组织移植到对侧卵巢和腹壁，建立了Sprague-Dawley大鼠子宫内膜异位症模型。注射Al18F-NOTA-FAPI-04后60分钟进行小动物PET/CT成像。定量分析显示，卵巢子宫内膜异位症病变(OELs；SUVmax = 2.53±0.61)，与相邻非病变卵巢组织(sOEL；（1.28±0.46,P = 0.031）和对侧卵巢组织(CO；1.15±0.49,p = 0.016)。同样，腹膜子宫内膜异位症病变（PELs）相对于周围腹膜子宫内膜异位病变(sPEL；0.97±0.13,P = 0.001)和腹壁肌(AWM；0.83±0.12,p = 0.0004)。组织病理学和免疫组织化学分析进一步证实了影像学结果。这些结果表明，Al18F-NOTA-FAPI-04 PET/CT可以有效区分子宫内膜异位症病变与正常组织，突出了其作为子宫内膜异位症无创诊断工具的潜力。

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来源期刊

Journal of Radiation Research and Applied Sciences MULTIDISCIPLINARY SCIENCES-

自引率

5.90%

发文量

130

审稿时长

16 weeks

期刊介绍： Journal of Radiation Research and Applied Sciences provides a high quality medium for the publication of substantial, original and scientific and technological papers on the development and applications of nuclear, radiation and isotopes in biology, medicine, drugs, biochemistry, microbiology, agriculture, entomology, food technology, chemistry, physics, solid states, engineering, environmental and applied sciences.