ELF18-INDUCED LONG NONCODING RNA 11 negatively regulates the innate immune response by suppressing Pathogenesis-related Lipase 5

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-06-06 DOI:10.1016/j.plaphy.2025.110138

Jimin Lee , Nuri Oh , Moonhyuk Kwon , Jun Sung Seo , Jang-Kyun Seo , Jin-Ho Kang , Hye Sun Cho , Choonkyun Jung

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引用次数: 0

Abstract

Long noncoding RNAs (lncRNAs) are essential components of diverse biological processes. However, lncRNAs involved in the innate immune response remain poorly unknown in plants. Here, we isolated ten lncRNAs named ELF18-INDUCED LONG NONCODING RNA (ELENA2 to 11) from custom lncRNA array datasets generated from Arabidopsis seedlings treated with pathogen-associated molecular patterns (PAMPs; elf18 and flg22). Each ELENA exhibited different expression patterns in a tissue- and PAMP-specific manner. ELENA11 is an intergenic lncRNA that is located between AT5G24170 and Pathogenesis-Related Lipase 5 (PRLIP5; AT5G24180). ELENA11-overexpressing plants showed reduced PRLIP5 expression and callose deposition compared to wild-type plants under elf18 and flg22 treatments, whereas ELENA11 knockout plants showed enhanced PRLIP5 expression and callose deposition, indicating that ELENA11 acts as a negative regulator of PRLIP5 expression. In addition, ELENA11-overexpressing plants were more susceptible to disease caused by Pseudomonas syringae pv. tomato DC3000 than wild-type plants, whereas ELENA11 knockout plants were resistant. These results demonstrate that ELENA11 plays a distinct role in PAMP-triggered innate immune responses by repressing PRLIP5 expression.

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elf18诱导的长链非编码RNA 11通过抑制发病相关的脂肪酶5负向调节先天免疫反应

长链非编码rna （lncRNAs）是多种生物过程的重要组成部分。然而，在植物中参与先天免疫反应的lncrna仍然知之甚少。在这里，我们从自定义lncRNA阵列数据集中分离了10个命名为elf18诱导的长非编码RNA （ELENA2至11）的lncRNA，这些数据集来自经病原体相关分子模式（PAMPs）处理的拟南芥幼苗。Elf18和flg22)。每个ELENA在组织和pamp特异性方面表现出不同的表达模式。ELENA11是一种基因间lncRNA，位于AT5G24170和发病相关脂肪酶5 (PRLIP5；AT5G24180)。ELENA11过表达植株与野生型植株相比，在elf18和flg22处理下，PRLIP5的表达和胼质沉积减少，而ELENA11敲除植株PRLIP5的表达和胼质沉积增加，表明ELENA11对PRLIP5的表达起负调控作用。此外，elena11过表达的植株更容易受到丁香假单胞菌的感染。而ELENA11基因敲除植株对番茄DC3000的抗性较强。这些结果表明，ELENA11通过抑制PRLIP5的表达，在pamp触发的先天免疫应答中发挥着独特的作用。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.