An ALOG transcription factor targets a TALE homeobox gene during corolla abscission in Torenia fournieri

IF 8.3 1区 生物学 Q1 PLANT SCIENCES
New Phytologist Pub Date : 2025-06-09 DOI:10.1111/nph.70291
Jiahuizi Li, Binghou Li, Xuan Zhou, Xiaofang Ma, Ruohan Xie, Yufei Sun, Shihao Su
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引用次数: 0

Abstract

Summary The ALOG genes encode plant‐specific transcription factors with conserved yet partially divergent functions across the plant kingdom. However, few direct downstream targets of ALOG transcription factors have been characterized to date. Here, we investigated the ALOG protein TfALOG3 in Torenia fournieri and identified its novel role through DNA affinity purification sequencing (DAP‐seq), transcriptome sequencing (RNA‐seq), and biochemical assays. We further validated a direct downstream target of TfALOG3 using RNA in situ hybridization and CRISPR/Cas9‐mediated gene editing. TfALOG3 was initially described as a key regulator of corolla development via its interaction with a BLADE‐ON‐PETIOLE orthologue, TfBOP2. Here, we demonstrate that the BOP‐ALOG complex also promotes corolla abscission. Furthermore, we identified a TALE homeobox gene, TfATH1, as a downstream target of TfALOG3. TfATH1 exhibits high expression in the proximal corolla region, and we show that TfALOG3 directly binds to the TfATH1 promoter to activate its transcription. Loss‐of‐function mutants of TfATH1 suggest that TfALOG3 acts upstream of TfATH1 and downregulation of TfATH1 expression leads to abnormal corolla abscission. Collectively, our findings reveal a previously uncharacterized role for an ALOG transcription factor and elucidate its mechanistic contribution to corolla abscission.
在花冠脱落过程中,ALOG转录因子靶向TALE同源盒基因
ALOG基因编码的植物特异性转录因子在整个植物界具有保守但部分分化的功能。然而,迄今为止,很少有ALOG转录因子的直接下游靶点被表征。在这里,我们研究了Torenia fournieri中的ALOG蛋白TfALOG3,并通过DNA亲和纯化测序(DAP‐seq)、转录组测序(RNA‐seq)和生化分析确定了它的新作用。我们使用RNA原位杂交和CRISPR/Cas9介导的基因编辑进一步验证了TfALOG3的直接下游靶点。TfALOG3最初被描述为花冠发育的关键调控因子,通过与叶片- ON -叶柄同源物TfBOP2相互作用。在这里,我们证明了BOP - ALOG复合物也促进花冠脱落。此外,我们确定了一个TALE同源盒基因TfATH1作为TfALOG3的下游靶标。TfATH1在近花冠区高表达,我们发现TfALOG3直接结合TfATH1启动子激活其转录。TfATH1的功能缺失突变表明,tfaog3作用于TfATH1的上游,TfATH1表达的下调导致花冠脱落异常。总的来说,我们的研究结果揭示了ALOG转录因子以前未被描述的作用,并阐明了其对花冠脱落的机制贡献。
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来源期刊
New Phytologist
New Phytologist 生物-植物科学
自引率
5.30%
发文量
728
期刊介绍: New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.
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