Jiahuizi Li, Binghou Li, Xuan Zhou, Xiaofang Ma, Ruohan Xie, Yufei Sun, Shihao Su
{"title":"An ALOG transcription factor targets a TALE homeobox gene during corolla abscission in Torenia fournieri","authors":"Jiahuizi Li, Binghou Li, Xuan Zhou, Xiaofang Ma, Ruohan Xie, Yufei Sun, Shihao Su","doi":"10.1111/nph.70291","DOIUrl":null,"url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item>The ALOG genes encode plant‐specific transcription factors with conserved yet partially divergent functions across the plant kingdom. However, few direct downstream targets of ALOG transcription factors have been characterized to date.</jats:list-item> <jats:list-item>Here, we investigated the ALOG protein TfALOG3 in <jats:italic>Torenia fournieri</jats:italic> and identified its novel role through DNA affinity purification sequencing (DAP‐seq), transcriptome sequencing (RNA‐seq), and biochemical assays. We further validated a direct downstream target of TfALOG3 using RNA <jats:italic>in situ</jats:italic> hybridization and CRISPR/Cas9‐mediated gene editing.</jats:list-item> <jats:list-item>TfALOG3 was initially described as a key regulator of corolla development via its interaction with a BLADE‐ON‐PETIOLE orthologue, TfBOP2. Here, we demonstrate that the BOP‐ALOG complex also promotes corolla abscission. Furthermore, we identified a TALE homeobox gene, <jats:italic>TfATH1</jats:italic>, as a downstream target of TfALOG3. <jats:italic>TfATH1</jats:italic> exhibits high expression in the proximal corolla region, and we show that TfALOG3 directly binds to the <jats:italic>TfATH1</jats:italic> promoter to activate its transcription. Loss‐of‐function mutants of <jats:italic>TfATH1</jats:italic> suggest that TfALOG3 acts upstream of <jats:italic>TfATH1</jats:italic> and downregulation of <jats:italic>TfATH1</jats:italic> expression leads to abnormal corolla abscission.</jats:list-item> <jats:list-item>Collectively, our findings reveal a previously uncharacterized role for an ALOG transcription factor and elucidate its mechanistic contribution to corolla abscission.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"19 1","pages":""},"PeriodicalIF":8.3000,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Phytologist","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/nph.70291","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
SummaryThe ALOG genes encode plant‐specific transcription factors with conserved yet partially divergent functions across the plant kingdom. However, few direct downstream targets of ALOG transcription factors have been characterized to date.Here, we investigated the ALOG protein TfALOG3 in Torenia fournieri and identified its novel role through DNA affinity purification sequencing (DAP‐seq), transcriptome sequencing (RNA‐seq), and biochemical assays. We further validated a direct downstream target of TfALOG3 using RNA in situ hybridization and CRISPR/Cas9‐mediated gene editing.TfALOG3 was initially described as a key regulator of corolla development via its interaction with a BLADE‐ON‐PETIOLE orthologue, TfBOP2. Here, we demonstrate that the BOP‐ALOG complex also promotes corolla abscission. Furthermore, we identified a TALE homeobox gene, TfATH1, as a downstream target of TfALOG3. TfATH1 exhibits high expression in the proximal corolla region, and we show that TfALOG3 directly binds to the TfATH1 promoter to activate its transcription. Loss‐of‐function mutants of TfATH1 suggest that TfALOG3 acts upstream of TfATH1 and downregulation of TfATH1 expression leads to abnormal corolla abscission.Collectively, our findings reveal a previously uncharacterized role for an ALOG transcription factor and elucidate its mechanistic contribution to corolla abscission.
期刊介绍:
New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.