Alleviating Effects of Vitamins E and D on Tamoxifen-Induced Hepatotoxicity in Female Wistar Rats.

Abstract

The protective effects of vitamins E and D, stemming from their antioxidant and anti-inflammatory capabilities were investigated in the context of tamoxifen-induced liver toxicity. The study involved twenty-five female rats divided into five experimental groups: group C (olive oil: 500 µL), group T (tamoxifen: 40 mg/kg), groups TE (tamoxifen: 40 mg/kg and vitamin E: 100 IU/kg), group TD (tamoxifen: 40 mg/kg and vitamin D: 500 IU/kg) and group TED (tamoxifen: 40 mg/kg, vitamin E: 100 IU/kg and vitamin D: 500 IU/kg). Calorimetric methods were used to measure biochemical variables, hepatic level of Total antioxidant capacity (TAC), Total oxidant status (TOS), Malondialdehyde (MDA), Superoxide dismutase (SOD), Catalase (CAT), and Glutathione peroxidase (GPx). Hepatic level of tumor necrosis factor-α (TNF-α) was assayed by ELISA. Hematoxylin-eosin staining method was also employed to assess Tamoxifen-induced liver lesions. Tamoxifen treatment resulted in a significant increase in ALP (p-value<0.01), TOS, and OSI (p-value<0.01), a marked decrease in TAC level (p-value<0.01), and an increase in TNF-α level. Simultaneous treatment with combined supplementation of vitamins E and D effectively stopped the decrease of TAC and the increase of TOS, OSI, and MDA. Although treatment with tamoxifen led to a decrease SOD, CAT, and GPX compared with group C, the difference wasn't significant. Treatment with vitamins considerably caused to increase SOD and CAT activity in comparison with groups C and T. The current study's findings demonstrated that the combined supplementation of vitamins E, and D effectively alleviated Tamoxifen-associated liver damage and oxidative stress.