Investigating RNA dynamics from single molecule transcriptomes.

IF 13.6 2区生物学 Q1 GENETICS & HEREDITY

Trends in Genetics Pub Date : 2025-06-04 DOI:10.1016/j.tig.2025.05.001

Sahiti Somalraju, Doaa Hassan Salem, Sarath Chandra Janga

引用次数: 0

Abstract

Investigating RNA dynamics is crucial for uncovering fundamental mechanisms, such as alternative splicing, RNA stability, and post-transcriptional modifications, all processes with implications for identifying therapeutic targets and advancing knowledge of cellular function and regulation. Advances in long-read sequencing technologies, particularly from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer unprecedented insights into RNA dynamics at single molecule and single nucleotide resolutions. In this review, we examine protocols and methods for analyzing RNA dynamics, focusing on isoform detection, poly(A) tail length quantification, and mapping of RNA modifications. We envision that these high-throughput, transcriptome-wide data sets, combined with integrated software systems, will transform workflows for studying single molecule RNA dynamics. Such advances will help unravel the complexities of gene regulation and deepen our understanding of cellular processes.

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从单分子转录组研究RNA动力学。

研究RNA动力学对于揭示诸如选择性剪接、RNA稳定性和转录后修饰等基本机制至关重要，所有这些过程都对确定治疗靶点和推进细胞功能和调控知识具有重要意义。长读测序技术的进步，特别是来自太平洋生物科学公司（PacBio）和牛津纳米孔技术公司（ONT）的进步，为单分子和单核苷酸分辨率的RNA动力学提供了前所未有的见解。在这篇综述中，我们研究了分析RNA动力学的协议和方法，重点是异构体检测，多聚(A)尾长定量和RNA修饰的定位。我们设想这些高通量，转录组范围的数据集，结合集成的软件系统，将改变研究单分子RNA动力学的工作流程。这些进展将有助于揭示基因调控的复杂性，加深我们对细胞过程的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Genetics 生物-遗传学

CiteScore

20.90

自引率

0.90%

发文量

160

审稿时长

6-12 weeks

期刊介绍： Launched in 1985, Trends in Genetics swiftly established itself as a "must-read" for geneticists, offering concise, accessible articles covering a spectrum of topics from developmental biology to evolution. This reputation endures, making TiG a cherished resource in the genetic research community. While evolving with the field, the journal now embraces new areas like genomics, epigenetics, and computational genetics, alongside its continued coverage of traditional subjects such as transcriptional regulation, population genetics, and chromosome biology. Despite expanding its scope, the core objective of TiG remains steadfast: to furnish researchers and students with high-quality, innovative reviews, commentaries, and discussions, fostering an appreciation for advances in genetic research. Each issue of TiG presents lively and up-to-date Reviews and Opinions, alongside shorter articles like Science & Society and Spotlight pieces. Invited from leading researchers, Reviews objectively chronicle recent developments, Opinions provide a forum for debate and hypothesis, and shorter articles explore the intersection of genetics with science and policy, as well as emerging ideas in the field. All articles undergo rigorous peer-review.