A Brief Guide to Analyzing TurboID-Based Proximity Labeling-Mass Spectrometry in Plants.

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecules and Cells Pub Date : 2025-06-03 DOI:10.1016/j.mocell.2025.100236

Tae-Ki Park, Tae-Wuk Kim

引用次数: 0

Abstract

Proximity labeling-mass spectrometry (PL-MS) has emerged as a powerful biological tool for elucidating the intracellular protein interactome of target proteins. Here, we introduce experimental strategies and methodologies for understanding complex protein-protein interactions in plant cells using the recently developed TurboID (TbID)-based PL approach. We provide a brief overview of the overall procedures, including the preparation of transgenic Arabidopsis plants, biotin treatment and affinity purification, on-bead digestion, LC-MS/MS, and data analysis.

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基于turboid的植物接近标记-质谱分析简介。

近距离标记-质谱法（PL-MS）已成为一种强大的生物学工具，用于阐明细胞内蛋白相互作用的目标蛋白。在这里，我们介绍了使用最近开发的基于TurboID （TbID）的PL方法来理解植物细胞中复杂蛋白质-蛋白质相互作用的实验策略和方法。我们简要概述了转基因拟南芥植株的制备、生物素处理和亲和纯化、头上消化、LC-MS/MS和数据分析等过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecules and Cells 生物-生化与分子生物学

CiteScore

6.60

自引率

10.50%

发文量

审稿时长

2.3 months

期刊介绍： Molecules and Cells is an international on-line open-access journal devoted to the advancement and dissemination of fundamental knowledge in molecular and cellular biology. It was launched in 1990 and ISO abbreviation is "Mol. Cells". Reports on a broad range of topics of general interest to molecular and cell biologists are published. It is published on the last day of each month by the Korean Society for Molecular and Cellular Biology.