Targeted Gene Delivery to MCF-7 Cells via Polyspermine-PEG-Glucose/DNA Nanoparticles: Preparation and Characterization.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biotechnology Pub Date : 2025-06-05 DOI:10.1007/s12033-025-01454-0

Sahar Mohajeri, Aytak Fathi Erdi, Hashem Yaghoubi

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引用次数: 0

Abstract

Cationic polymers are positively charged polymers that have a high ability to electrostatically interact with negatively charged species, including nucleic acids. This property has led to the use of these polymers in various fields, including targeted drug delivery. In this study, biocompatible polyspermine-polyethylene glycol (PEG)-glucose (PSPG) nanoparticles were synthesized for DNA delivery to MCF-7 cells. Structural characterization was performed via Fourier transform infrared spectroscopy (FTIR), hydrogen nuclear magnetic resonance spectroscopy (¹HNMR), thermogravimetric analysis (TGA), and differential thermal gravimetric (DTG) methods. The DNA-loaded nanoparticles exhibited a spherical morphology with smooth surfaces, as confirmed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Dynamic light scattering (DLS) revealed a particle size of 267 ± 10 nm and a surface charge of + 13.4 ± 1.3 mV. DNA release from PSPG was pH dependent, increasing at acidic pH (5.0, cancer cells) compared with physiological pH (7.4). The biocompatibility assessed via MTT demonstrated high gene transfer efficiency with minimal cytotoxicity. Agarose gel electrophoresis confirmed the protection of the DNA against enzymatic degradation. Gene delivery to MCF-7 cells was validated via fluorescence microscopy and flow cytometry, confirming successful transfection. These findings highlight the potential of polyspermine-PEG-glucose nanoparticles as efficient DNA carriers for targeted cancer therapy.

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通过聚精胺-聚乙二醇-葡萄糖/DNA纳米颗粒靶向基因递送MCF-7细胞：制备和表征。

阳离子聚合物是带正电的聚合物，具有与带负电的物质（包括核酸）静电相互作用的高能力。这种特性导致了这些聚合物在各种领域的使用，包括靶向药物输送。在这项研究中，合成了生物相容性的聚精胺-聚乙二醇-葡萄糖（PSPG）纳米颗粒，用于将DNA递送到MCF-7细胞。通过傅里叶变换红外光谱（FTIR）、氢核磁共振光谱（1HNMR）、热重分析（TGA）和差热重分析（DTG）方法进行了结构表征。通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）证实，负载dna的纳米颗粒呈球形，表面光滑。动态光散射（DLS）结果表明，该材料的粒径为267±10 nm，表面电荷为+ 13.4±1.3 mV。PSPG的DNA释放是pH依赖性的，与生理pH（7.4）相比，酸性pH（5.0，癌细胞）增加。通过MTT评估的生物相容性显示出高的基因转移效率和最小的细胞毒性。琼脂糖凝胶电泳证实了DNA对酶降解的保护作用。通过荧光显微镜和流式细胞术验证基因传递到MCF-7细胞，证实转染成功。这些发现突出了聚精胺-聚乙二醇-葡萄糖纳米颗粒作为靶向癌症治疗的有效DNA载体的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.