Crystal Structure of Camel (Camelus dromedarius) Methemoglobin: A High Oxygen Affinity Lowland Species.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biotechnology Pub Date : 2025-06-05 DOI:10.1007/s12033-025-01456-y

Balasubramanian Moovarkumudalvan, Sathya Moorthy Ponnuraj, Ramya Ramadoss, Thenmozhi Muthurajan, M N Ponnuswamy

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Abstract

Hemoglobin (Hb) is an important oxygen carrying protein inherent in erythrocytes facilitates exchange of oxygen and carbon dioxide from body tissues. Bioengineered synthetic erythrocytes with enhanced Hb proteins have been developed as efficient blood transfusion substitutes to supply oxygen to patients in extreme situations. This has driven research towards the molecular characterization and structural elucidation of Hb in organisms thriving in extreme conditions. Camel is recognized as a typical organism to investigate molecular level adaptations to surpass extreme ecological conditions in the arid zone. The molecular architecture of Hb is linked to the oxygen requirements of an organism to sustain at extreme living conditions. Molecular structure of camel Hb can be determined by X-ray crystallography. In this study, camel Hb is purified and crystallized in the monoclinic space group P2₁ with cell dimensions of a = 52.75 Å, b = 116.78 Å, c = 52.80 Å and β = 120.07 Å. The crystal packing parameters reveal that a whole biological molecule is present in the asymmetric unit with the final R-factor and R_free value 23.1 and 29.5%, respectively. The Quaternary structural analysis of the camel Hb [PDB ID: 3GDJ] suggests its state to be identical to human R-state Hb.

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骆驼（Camelus dromedarius）高氧亲和性低地物种高铁血红蛋白的晶体结构。

血红蛋白（Hb）是红细胞中重要的载氧蛋白，促进身体组织中氧气和二氧化碳的交换。具有增强Hb蛋白的生物工程合成红细胞已被开发为有效的输血替代品，为极端情况下的患者提供氧气。这推动了在极端条件下蓬勃发展的生物体中Hb的分子表征和结构阐明的研究。骆驼被认为是研究干旱区极端生态条件下分子水平适应的典型生物。血红蛋白的分子结构与生物体在极端生活条件下维持所需的氧气有关。用x射线晶体学可以测定驼鹿血红蛋白的分子结构。在本研究中，骆驼Hb被纯化并在单斜空间组P21中结晶，细胞尺寸为a = 52.75 Å， b = 116.78 Å， c = 52.80 Å和β = 120.07 Å。晶体填充参数表明，不对称单元中存在一个完整的生物分子，最终r因子和Rfree值分别为23.1和29.5%。对骆驼Hb的第四纪结构分析[PDB ID: 3GDJ]表明其状态与人类r -状态Hb相同。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.