Expressions of IGF-1, pAKT, and pS6 in Uterosacral Ligaments of Patients with Pelvic Organ Prolapse.

IF 1.8 3区 医学 Q3 OBSTETRICS & GYNECOLOGY
Hongling Xu, Gensheng Wang, Qing Li, Xuemei Wang, Dongdi Xu
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Abstract

Introduction and hypothesis: The objective was to investigate the expressions and significance of IGF-1, pAKT, pS6, and type I and type III collagens in uterosacral ligaments of patients with pelvic organ prolapse (POP).

Methods: A total of 38 patients with POP who underwent hysterectomy or pelvic floor reconstruction were selected as the POP group. Another 38 patients who underwent hysterectomy owing to nonpelvic floor dysfunction were selected as the control group. The POP quantitative staging scores of the two groups were III-IV and 0-I respectively. The histological manifestations of the abandoned uterosacral ligament tissues near the cervix were evaluated using hematoxylin-eosin staining and Masson staining. The expressions of IGF-1, plGF-1R, pAKT, pS6, and type I and type III collagen fibers were detected using immunohistochemistry and Western blotting.

Results: Hematoxylin-eosin and Masson staining showed that the number of POP constituent fibrocytes decreased, the arrangement was disordered, and the distribution of collagen fibers was sparse compared with the control group. Immunohistochemical analysis showed that the expressions of IGF-1, pIGF-1R, pAKT , pS6, and type I and type III collagen in uterosacral ligament tissues of the POP group significantly decreased. Western blotting demonstrated that the expressions of IGF-1, pIGF-1R, pAKT, pS6, and type I and type III collagen in the POP group significantly decreased.

Conclusion: The expressions of IGF-1, pAKT, pS6, and type I and type III collagen decreased in the uterosacral ligament tissues of POP patients, and the IGF-1/AKT/mTORC1 signaling pathway regulated by IGF-1 may be related to the occurrence of POP.

IGF-1、pAKT和pS6在盆腔器官脱垂患者子宫骶韧带中的表达
前言与假设:目的探讨IGF-1、pAKT、pS6、I型和III型胶原在盆腔器官脱垂(POP)患者子宫骶韧带中的表达及其意义。方法:选择行子宫切除术或盆底重建的POP患者38例作为POP组。另外38例因非盆底功能障碍而行子宫切除术的患者作为对照组。两组患者的POP定量分期评分分别为III-IV分和0-I分。采用苏木精-伊红染色和马松染色评价宫颈附近废弃子宫骶韧带组织的组织学表现。免疫组织化学和Western blotting检测IGF-1、plGF-1R、pAKT、pS6、I型和III型胶原纤维的表达。结果:苏木精-伊红和马松染色显示,与对照组相比,POP组成纤维细胞数量减少,排列紊乱,胶原纤维分布稀疏。免疫组化分析显示,POP组子宫骶韧带组织中IGF-1、pIGF-1R、pAKT、pS6、I型和III型胶原蛋白表达明显降低。Western blotting结果显示,POP组IGF-1、pIGF-1R、pAKT、pS6、I型和III型胶原蛋白的表达明显降低。结论:POP患者子宫骶韧带组织中IGF-1、pAKT、pS6、I型和III型胶原蛋白表达降低,IGF-1调控的IGF-1/AKT/mTORC1信号通路可能与POP的发生有关。
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来源期刊
CiteScore
3.80
自引率
22.20%
发文量
406
审稿时长
3-6 weeks
期刊介绍: The International Urogynecology Journal is the official journal of the International Urogynecological Association (IUGA).The International Urogynecology Journal has evolved in response to a perceived need amongst the clinicians, scientists, and researchers active in the field of urogynecology and pelvic floor disorders. Gynecologists, urologists, physiotherapists, nurses and basic scientists require regular means of communication within this field of pelvic floor dysfunction to express new ideas and research, and to review clinical practice in the diagnosis and treatment of women with disorders of the pelvic floor. This Journal has adopted the peer review process for all original contributions and will maintain high standards with regard to the research published therein. The clinical approach to urogynecology and pelvic floor disorders will be emphasized with each issue containing clinically relevant material that will be immediately applicable for clinical medicine. This publication covers all aspects of the field in an interdisciplinary fashion
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