Recovery and protection of photo-damaged hairs with keratin-encapsulated cationic liposomes

IF 2.5 4区医学 Q2 DERMATOLOGY

International Journal of Cosmetic Science Pub Date : 2025-06-06 DOI:10.1111/ics.13080

Hoai-Thuong Duc Bui, Su Ji Kim, Jihui Jang, Jun Bae Lee, Hyuk Sang Yoo

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引用次数: 0

Abstract

Objective

Daily sunlight exposure impacts the structural integrity and chemical composition of hair in both reversible and permanent manners. Although keratin-based treatments have been popularly used to repair damaged hair, their effects in the recovery of the hair damage caused by UV light have not been well studied. Moreover, limitations related to keratin penetration depth and duration of effect persist. Encapsulating active ingredients in liposomes has been shown to enhance their stability, bioavailability and permeability. Thus, we aimed to design cationic liposomes encapsulated with keratin to enhance the recovery effects of photo-damaged hair.

Methods

We prepared keratin-incorporated cationic liposomes (KLs) via high-pressure homogenization and assessed their physical properties via hydrodynamic size, zeta potential, electron microscopy and X-ray diffraction analyses. The physical and chemical damage level of hair samples before and after treatment, such as hair smoothness, protein degradation and lipid peroxidation, were subsequently investigated using electron microscopy and different spectroscopies.

Results

The results indicated that the cationic liposomes fabricated by high-pressure homogenization method had combined unilamellar and multilamellar structures. Compared with the keratin solution, the KL suspension significantly improved the permeation of keratin into the cortex during a 24-h incubation. Moreover, 24 and 48-h UV-exposed hairs showed enhanced recovery when incubated with keratin liposomes for 24 h, as evidenced by the observation of a smoother hair surface using electron microscopy. We observed that treatment with keratin liposomes significantly reduced protein denaturation and lipid peroxidation in the photo-damaged hairs.

Conclusion

We anticipate that cationic liposome-assisted keratin delivery may serve as an effective method for restoring photo-damaged hair in both the physical and chemical dimensions.

Abstract Image

查看原文本刊更多论文

角蛋白包封阳离子脂质体对光损伤头发的修复与保护。

目的：日常阳光照射对头发的结构完整性和化学成分的影响既有可逆的，也有永久的。虽然以角蛋白为基础的治疗方法已被广泛用于修复受损的头发，但它们对紫外线造成的头发损伤的恢复效果还没有得到很好的研究。此外，与角蛋白渗透深度和作用持续时间有关的限制仍然存在。在脂质体中包封活性成分已被证明可以提高它们的稳定性、生物利用度和渗透性。因此，我们旨在设计角蛋白包裹的阳离子脂质体来增强光损伤头发的恢复效果。方法：采用高压均质法制备角蛋白阳离子脂质体（KLs），并通过流体力学尺寸、zeta电位、电子显微镜和x射线衍射分析对其物理性质进行评价。随后，利用电子显微镜和不同光谱研究了处理前后头发样品的物理和化学损伤水平，如头发光滑度、蛋白质降解和脂质过氧化。结果：高压均质法制备的阳离子脂质体具有单层和多层结构。与角蛋白溶液相比，在24小时的孵育过程中，KL悬浮液显著提高了角蛋白向皮质的渗透。此外，当角蛋白脂质体孵育24小时时，暴露在紫外线下24和48小时的毛发恢复增强，电镜观察到毛发表面更光滑。我们观察到，角蛋白脂质体处理显著减少了光损伤头发中的蛋白质变性和脂质过氧化。结论：我们预计阳离子脂质体辅助角蛋白递送可能在物理和化学方面成为修复光损伤头发的有效方法。

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来源期刊

International Journal of Cosmetic Science DERMATOLOGY-

CiteScore

4.60

自引率

4.30%

发文量

期刊介绍： The Journal publishes original refereed papers, review papers and correspondence in the fields of cosmetic research. It is read by practising cosmetic scientists and dermatologists, as well as specialists in more diverse disciplines that are developing new products which contact the skin, hair, nails or mucous membranes. The aim of the Journal is to present current scientific research, both pure and applied, in: cosmetics, toiletries, perfumery and allied fields. Areas that are of particular interest include: studies in skin physiology and interactions with cosmetic ingredients, innovation in claim substantiation methods (in silico, in vitro, ex vivo, in vivo), human and in vitro safety testing of cosmetic ingredients and products, physical chemistry and technology of emulsion and dispersed systems, theory and application of surfactants, new developments in olfactive research, aerosol technology and selected aspects of analytical chemistry.