The Ran GTPase inhibits SGIV and RGNNV infection by upregulating host immune response in grouper

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-06-04 DOI:10.1016/j.fsi.2025.110479

Xiaozhi Wen , Lingfeng Guan , Liqun Wang , Zihan Zhang , Xinyan Wei , Qiwei Qin , Shaowen Wang

{"title":"The Ran GTPase inhibits SGIV and RGNNV infection by upregulating host immune response in grouper","authors":"Xiaozhi Wen , Lingfeng Guan , Liqun Wang , Zihan Zhang , Xinyan Wei , Qiwei Qin , Shaowen Wang","doi":"10.1016/j.fsi.2025.110479","DOIUrl":null,"url":null,"abstract":"<div><div>Ran is a small G-protein that acts as a “molecular switch” in nucleoplasmic transport regulating cellular activities. However, the functions of Ran in grouper and their effects on the viral infections are still unclear. In this study, we identified and characterized Ran in <em>Epinephelus coioides</em> (EcRan). EcRan encodes a 215 amino acid polypeptide containing key conserved domains including G1-G5 box and C terminal domains. EcRan was widely expressed in different tissues of healthy groupers, and showed obvious nucleus localization. Upon infection of Singapore grouper iridovirus (SGIV) or red spotted grouper nervous necrosis virus (RGNNV), EcRan transcript was significantly decreased. Moreover, overexpression of EcRan remarkably inhibited the replication of SGIV and RGNNV, whereas knockdown of EcRan notably promoted the replication of SGIV and RGNNV. In addition, overexpressed EcRan positively regulated the transcription of interferon (IFN)-related and inflammatory factors, including IFN regulatory factor 3 (IRF3), myxovirus resistance gene 1 (MXI), laboratory of genetics and physiology 2 (LGP2), tumor necrosis factor alpha (TNF-α), tumor necrosis factor receptor-associated factor 6 (TRAF6) and interleukin 8 (IL-8). The transcription of these immune genes was down regulated when EcRan transcription was inhibited by siRNA. Taken together, EcRan showed the antiviral effects against SGIV and RGNNV infections by positively regulating innate immune response.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"165 ","pages":"Article 110479"},"PeriodicalIF":4.1000,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fish & shellfish immunology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1050464825003687","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}

引用次数: 0

Abstract

Ran is a small G-protein that acts as a “molecular switch” in nucleoplasmic transport regulating cellular activities. However, the functions of Ran in grouper and their effects on the viral infections are still unclear. In this study, we identified and characterized Ran in Epinephelus coioides (EcRan). EcRan encodes a 215 amino acid polypeptide containing key conserved domains including G1-G5 box and C terminal domains. EcRan was widely expressed in different tissues of healthy groupers, and showed obvious nucleus localization. Upon infection of Singapore grouper iridovirus (SGIV) or red spotted grouper nervous necrosis virus (RGNNV), EcRan transcript was significantly decreased. Moreover, overexpression of EcRan remarkably inhibited the replication of SGIV and RGNNV, whereas knockdown of EcRan notably promoted the replication of SGIV and RGNNV. In addition, overexpressed EcRan positively regulated the transcription of interferon (IFN)-related and inflammatory factors, including IFN regulatory factor 3 (IRF3), myxovirus resistance gene 1 (MXI), laboratory of genetics and physiology 2 (LGP2), tumor necrosis factor alpha (TNF-α), tumor necrosis factor receptor-associated factor 6 (TRAF6) and interleukin 8 (IL-8). The transcription of these immune genes was down regulated when EcRan transcription was inhibited by siRNA. Taken together, EcRan showed the antiviral effects against SGIV and RGNNV infections by positively regulating innate immune response.

查看原文本刊更多论文

Ran GTPase通过上调宿主免疫反应抑制石斑鱼SGIV和RGNNV感染。

Ran是一种小的g蛋白，在核质运输中起着调节细胞活动的“分子开关”作用。然而，Ran在石斑鱼体内的功能及其对病毒感染的影响尚不清楚。本研究对石斑鱼（Epinephelus coioides, EcRan）中的Ran进行了鉴定和鉴定。EcRan编码一个215个氨基酸的多肽，包含G1-G5盒和C端结构域。EcRan在健康石斑鱼的不同组织中广泛表达，并表现出明显的核定位。感染新加坡石斑鱼虹膜病毒（SGIV）或红斑石斑鱼神经坏死病毒（RGNNV）后，EcRan转录量显著降低。此外，过表达EcRan显著抑制SGIV和RGNNV的复制，而低表达EcRan则显著促进SGIV和RGNNV的复制。此外，过表达的EcRan正调控干扰素（IFN）相关因子和炎症因子的转录，包括干扰素调节因子3 （IRF3）、黏液病毒抗性基因1 （MXI）、遗传生理实验室2 （LGP2）、肿瘤坏死因子α (TNF-α)、肿瘤坏死因子受体相关因子6 （TRAF6）和白细胞介素8 （IL-8）。当siRNA抑制EcRan转录时，这些免疫基因的转录下调。综上所述，EcRan通过积极调节先天免疫反应，显示出对SGIV和RGNNV感染的抗病毒作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.