Embryonic cerebrospinal fluid pressure in fetal mice in utero: External factors pressurize the intraventricular space.

IF 2 3区生物学 Q2 ANATOMY & MORPHOLOGY

Developmental Dynamics Pub Date : 2025-06-06 DOI:10.1002/dvdy.70047

Koichiro Tsujikawa, Reina Muramatsu, Takaki Miyata

{"title":"Embryonic cerebrospinal fluid pressure in fetal mice in utero: External factors pressurize the intraventricular space.","authors":"Koichiro Tsujikawa, Reina Muramatsu, Takaki Miyata","doi":"10.1002/dvdy.70047","DOIUrl":null,"url":null,"abstract":"Background: Previous experiments inducing leakage of embryonic cerebrospinal fluid (CSF) suggest the necessity of intraventricular CSF pressure (PCSF) for brain morphogenesis. Nevertheless, how embryonic PCSF occurs is unclear, especially in utero.Results: Using a Landis water manometer, we measured PCSF in fetal mice isolated from the amniotic cavity (PCSF-ISO) and found that PCSF-ISO rose from 20 Pa at embryonic day (E) 10 to 100-110 Pa at E14-16. At E13, intraventricular injections of ≥3 μL of saline elevated PCSF-ISO by ~30%, whereas those of inhibitors of CSF secretion decreased PCSF-ISO by ~30%. Shh-mediated cerebral wall expansion did not significantly increase PCSF-ISO. Removal of the brain-surrounding contractile tissues decreased PCSF-ISO by 80%-90%. We then found that the intraamniotic pressure measured in utero (PAF-IU) declined from 2000 Pa at E10 to 500 Pa at E15-18 but was always much greater than PCSF-ISO. Direct measurement of PCSF in utero (PCSF-IU) at E13 and E15 coupled with the measurement of PCSF-ISO under hydrostatic pressure loading to mimic PAF-IU at various embryonic ages revealed the following relationship: PCSF-IU = PCSF-ISO + PAF-IU.Conclusions: The PCSF of mice in utero is influenced by external factors, most strongly by intraamniotic pressure and less strongly by brain-confining tissues.","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":" ","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Developmental Dynamics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1002/dvdy.70047","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Previous experiments inducing leakage of embryonic cerebrospinal fluid (CSF) suggest the necessity of intraventricular CSF pressure (P_CSF) for brain morphogenesis. Nevertheless, how embryonic P_CSF occurs is unclear, especially in utero.

Results: Using a Landis water manometer, we measured P_CSF in fetal mice isolated from the amniotic cavity (P_CSF-ISO) and found that P_CSF-ISO rose from 20 Pa at embryonic day (E) 10 to 100-110 Pa at E14-16. At E13, intraventricular injections of ≥3 μL of saline elevated P_CSF-ISO by ~30%, whereas those of inhibitors of CSF secretion decreased P_CSF-ISO by ~30%. Shh-mediated cerebral wall expansion did not significantly increase P_CSF-ISO. Removal of the brain-surrounding contractile tissues decreased P_CSF-ISO by 80%-90%. We then found that the intraamniotic pressure measured in utero (P_AF-IU) declined from 2000 Pa at E10 to 500 Pa at E15-18 but was always much greater than P_CSF-ISO. Direct measurement of P_CSF in utero (P_CSF-IU) at E13 and E15 coupled with the measurement of P_CSF-ISO under hydrostatic pressure loading to mimic P_AF-IU at various embryonic ages revealed the following relationship: P_CSF-IU = P_CSF-ISO + P_AF-IU.

Conclusions: The P_CSF of mice in utero is influenced by external factors, most strongly by intraamniotic pressure and less strongly by brain-confining tissues.

查看原文本刊更多论文

胎鼠在子宫内的胚胎脑脊液压力：外部因素使脑室内空间受压。

背景：以往诱导胚胎脑脊液（CSF）渗漏的实验提示脑室内CSF压力（PCSF）是脑形态发生的必要条件。然而，胚胎PCSF是如何发生的尚不清楚，特别是在子宫内。结果：利用Landis水压力计测定羊膜腔分离胎鼠的PCSF (PCSF- iso)，发现PCSF- iso从胚胎日(E) 10时的20 Pa上升到E14-16时的100-110 Pa。在E13时，脑室内注射≥3 μL生理盐水使PCSF-ISO升高约30%，而脑脊液分泌抑制剂使PCSF-ISO降低约30%。sh介导的脑壁扩张未显著增加PCSF-ISO。切除脑周围的收缩组织可使PCSF-ISO降低80%-90%。然后我们发现子宫内测量的羊膜内压（PAF-IU）从E10时的2000 Pa下降到E15-18时的500 Pa，但总是远高于PCSF-ISO。在E13和E15直接测量子宫内PCSF (PCSF- iu)，并在静水压力加载下测量PCSF- iso，模拟不同胎龄的PAF-IU，结果显示：PCSF- iu = PCSF- iso + PAF-IU。结论：小鼠宫内PCSF受外界因素影响较大，受羊膜内压影响较大，受围脑组织影响较小。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Developmental Dynamics 生物-发育生物学

CiteScore

5.10

自引率

8.00%

发文量

116

审稿时长

3-8 weeks

期刊介绍： Developmental Dynamics, is an official publication of the American Association for Anatomy. This peer reviewed journal provides an international forum for publishing novel discoveries, using any model system, that advances our understanding of development, morphology, form and function, evolution, disease, stem cells, repair and regeneration.