Antibacterial Activity and Mechanistic Insights into Bioinspired Hydrophilic Selenium-Iron-Sulfur Hybrid (Se-S-Fe) Nanostructures.

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2025-06-06 DOI:10.1021/acsbiomaterials.5c00518

Shubhangi D Shirsat, Chunyi Li, Zhipeng Liu, Varenyam Achal, Olivier Habimana

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Abstract

Hybrid nanoparticles (HNPs) offer integrated advantages in comparison to the singular-component systems of nanomaterials. This study reports a simple, one-pot green synthesis of hydrophilic selenium-iron-sulfur hybrid nanoparticles (Se-S-Fe HNPs) using an Alstonia scholaris extract. The size and surface charge of the Se-S-Fe HNPs, characterized by advanced material characterization techniques, significantly influenced their antimicrobial activity against Escherichia coli and Bacillus megaterium. However, mechanistic studies uncovered distinct modes of action against these bacterial species. Transcriptomic analysis revealed Se-S-Fe HNPs disrupted protein synthesis in E. coli and elevated the expression of outer membrane proteins OmpA and OmpC. In B. megaterium, the HNPs induced hyperosmotic shock and broad metabolic changes, impacting amino acid biosynthesis and protein localization. This work introduces a facile and environmentally friendly method for producing effective antimicrobial nanomaterials with distinct mechanisms of action depending on bacterial species.

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仿生亲水性硒-铁-硫杂化（Se-S-Fe）纳米结构的抗菌活性和机理研究。

与单组分纳米材料系统相比，混合纳米颗粒（HNPs）具有综合优势。本研究报道了一种简单的、一锅绿色合成亲水性硒-铁-硫杂化纳米粒子（Se-S-Fe HNPs）的方法。采用先进的材料表征技术对Se-S-Fe HNPs的大小和表面电荷进行了表征，结果表明Se-S-Fe HNPs对大肠杆菌和巨芽孢杆菌的抑菌活性有显著影响。然而，机制研究揭示了对抗这些细菌的不同作用模式。转录组学分析显示Se-S-Fe HNPs破坏了大肠杆菌蛋白的合成，提高了外膜蛋白OmpA和OmpC的表达。在megaterium中，HNPs诱导高渗透休克和广泛的代谢变化，影响氨基酸的生物合成和蛋白质的定位。这项工作介绍了一种简单和环保的方法来生产有效的抗菌纳米材料，根据细菌种类具有不同的作用机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

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