Alterations in the intestinal fungal microbiome of patients with severe fever with thrombocytopenia syndrome

IF 4.7 3区医学 Q1 INFECTIOUS DISEASES

Journal of Infection and Public Health Pub Date : 2025-05-23 DOI:10.1016/j.jiph.2025.102837

Meng-Yu Liu , Sheng-Fu He , Yu-Yao Li , Jiao-Jiao Shen , Jia-Jia Li , Ya-Sheng Li , Yan-Yan Liu , Ting Wu , Jia-Bin Li , Li-Fen Hu

{"title":"Alterations in the intestinal fungal microbiome of patients with severe fever with thrombocytopenia syndrome","authors":"Meng-Yu Liu , Sheng-Fu He , Yu-Yao Li , Jiao-Jiao Shen , Jia-Jia Li , Ya-Sheng Li , Yan-Yan Liu , Ting Wu , Jia-Bin Li , Li-Fen Hu","doi":"10.1016/j.jiph.2025.102837","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div><em>Aspergillus</em> coinfection in patients with severe fever with thrombocytopenia syndrome (SFTS), is a serious clinical challenge associated with increased mortality. Understanding the factors contributing to this co-infection is crucial for improving patient outcomes. This study aimed to reveal the role of the intestinal mycobiome in SFTS severity and the risk of <em>Aspergillus</em> coinfection, with the goal of identifying potential therapeutic targets.</div></div><div><h3>Methods</h3><div>Fecal samples were collected from 80 patients both during their hospitalization and post-discharge. Internal transcribed spacer (ITS) amplicon sequencing and fungal profiling of intestine were performed. R statistical software (version 3.5.1) was used for data processing and analysis.</div></div><div><h3>Results</h3><div>The intestinal mycobiomes of SFTS patients showed strong alterations characterized by increased <em>Aspergillus</em> species, and a highly heterogeneous mycobiome conﬁguration compared to healthy controls. The <em>Aspergillus</em> had a positive correlation with coinfection of invasive pulmonary aspergillosis (IPA) and disease severity of SFTS (<em>p</em> < 0.001), whereas <em>Saccharomycetales</em> and <em>Candida</em> were more abundant in SFTS patients without IPA (<em>p</em> < 0.001). In SFTS patients with IPA, <em>A. subversicolor</em>, <em>A. flavus</em> and <em>A. penicillioides</em> were the three most common fungal species. Longitudinal dynamic detection revealed that patients who experienced significant fluctuations in their intestinal mycobiome tended to have more severe illness. After recovering, the gut mycobiome of patients can recover and stabilize within a month.</div></div><div><h3>Conclusion</h3><div>The research highlighted enrichment of intestinal <em>Aspergillus</em> was conducive to IPA and disease severity in SFTS patients. Monitoring the gut mycobiome could potentially be used as a biomarker to assess disease severity of SFTS.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"18 9","pages":"Article 102837"},"PeriodicalIF":4.7000,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Infection and Public Health","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1876034125001868","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Aspergillus coinfection in patients with severe fever with thrombocytopenia syndrome (SFTS), is a serious clinical challenge associated with increased mortality. Understanding the factors contributing to this co-infection is crucial for improving patient outcomes. This study aimed to reveal the role of the intestinal mycobiome in SFTS severity and the risk of Aspergillus coinfection, with the goal of identifying potential therapeutic targets.

Methods

Fecal samples were collected from 80 patients both during their hospitalization and post-discharge. Internal transcribed spacer (ITS) amplicon sequencing and fungal profiling of intestine were performed. R statistical software (version 3.5.1) was used for data processing and analysis.

Results

The intestinal mycobiomes of SFTS patients showed strong alterations characterized by increased Aspergillus species, and a highly heterogeneous mycobiome conﬁguration compared to healthy controls. The Aspergillus had a positive correlation with coinfection of invasive pulmonary aspergillosis (IPA) and disease severity of SFTS (p < 0.001), whereas Saccharomycetales and Candida were more abundant in SFTS patients without IPA (p < 0.001). In SFTS patients with IPA, A. subversicolor, A. flavus and A. penicillioides were the three most common fungal species. Longitudinal dynamic detection revealed that patients who experienced significant fluctuations in their intestinal mycobiome tended to have more severe illness. After recovering, the gut mycobiome of patients can recover and stabilize within a month.

Conclusion

The research highlighted enrichment of intestinal Aspergillus was conducive to IPA and disease severity in SFTS patients. Monitoring the gut mycobiome could potentially be used as a biomarker to assess disease severity of SFTS.

查看原文本刊更多论文

重症发热伴血小板减少综合征患者肠道真菌微生物组的改变

背景：严重发热伴血小板减少综合征（SFTS）患者的曲霉合并感染是一个严重的临床挑战，与死亡率增加相关。了解导致这种合并感染的因素对于改善患者预后至关重要。本研究旨在揭示肠道菌群在SFTS严重程度和曲霉菌合并感染风险中的作用，以确定潜在的治疗靶点。方法收集80例患者住院期间及出院后的粪便标本。进行了肠道内转录间隔子（ITS）扩增子测序和真菌谱分析。采用R统计软件（3.5.1版）对数据进行处理和分析。结果与健康对照相比，SFTS患者肠道菌群表现出强烈的变化，其特征是曲霉菌种类增加，菌群结构高度异质性。曲霉菌与合并感染侵袭性肺曲霉病（IPA）和SFTS病情严重程度呈正相关（p <； 0.001），而Saccharomycetales和Candida在无IPA的SFTS患者中更为丰富（p <； 0.001）。在伴有IPA的SFTS患者中，浅色单胞菌、黄单胞菌和盘尼西林单胞菌是最常见的3种真菌。纵向动态检测显示，肠道菌群显著波动的患者往往有更严重的疾病。患者康复后，肠道菌群可在一个月内恢复稳定。结论肠道曲霉的富集有利于SFTS患者IPA的改善和病情的恶化。监测肠道菌群可能被用作评估SFTS疾病严重程度的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Infection and Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH -INFECTIOUS DISEASES

CiteScore

13.10

自引率

1.50%

发文量

203

审稿时长

96 days

期刊介绍： The Journal of Infection and Public Health, first official journal of the Saudi Arabian Ministry of National Guard Health Affairs, King Saud Bin Abdulaziz University for Health Sciences and the Saudi Association for Public Health, aims to be the foremost scientific, peer-reviewed journal encompassing infection prevention and control, microbiology, infectious diseases, public health and the application of healthcare epidemiology to the evaluation of health outcomes. The point of view of the journal is that infection and public health are closely intertwined and that advances in one area will have positive consequences on the other. The journal will be useful to all health professionals who are partners in the management of patients with communicable diseases, keeping them up to date. The journal is proud to have an international and diverse editorial board that will assist and facilitate the publication of articles that reflect a global view on infection control and public health, as well as emphasizing our focus on supporting the needs of public health practitioners. It is our aim to improve healthcare by reducing risk of infection and related adverse outcomes by critical review, selection, and dissemination of new and relevant information in the field of infection control, public health and infectious diseases in all healthcare settings and the community.