Structural regulation of carboxymethyl starch/whey protein isolated coacervate microcapsules for colon-targeted delivery of oleoylethanolamine and promotion of satiety hormones secretion

IF 11 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids Pub Date : 2025-05-30 DOI:10.1016/j.foodhyd.2025.111601

Haize Su , Yingying Li , Zhibing Zhang , Xiaoxi Li

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Abstract

Appetite regulation and weight control can be achieved by dietary modulation for the secretion of satiety hormones glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) from colonic L cells. Herein, to construct an oral colon-targeted delivery system, carboxymethyl starch (CMS)/denatured whey protein isolate (DWPI) coacervate microcapsules (CDCMs) were fabricated by encapsulating the oleoylethanolamide (OEA). Moreover, tannic acid was used as a crosslinker (CL) to adjust the interaction between the components, forming a more compact and ordered structure, with particle sizes ranging from 12.36 μm–13.30 μm, and OEA loading capacities ranging from 240.34 μg/mL ∼259.34 μg/mL. As the amount of CL was increased, CDCMs demonstrated an excellent ability to maintain structural integrity in the simulated upper gastrointestinal tract, with the degradation rates of CMS and DWPI decreasing from 30.53 % to 65.04 %–8.67 % and 26.33 %, respectively, and the release rate of OEA decreasing from 88.18 % to 20.61 %, showing a good colonic-targeted delivery performance. Furthermore, CDCM-1 fermentation in the colon increased the abundance of gut bacteria, especially the short chain fatty acids (SCFAs) producing bacteria. In addition, the sustained release of OEA (release of 24.34 mg/mL after 48h) synergized with CMS/DWPI metabolites such as reducing sugars, protein hydrolysates, and SCFAs effectively modulated GLP-1 and PYY secretion from L cells, with the highest releases of 28.03 pg/mL and 45.50 pg/mL at 48 h, respectively. Therefore, the prepared CDCMs have potential to be a prospective food for weight control.

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羧甲基淀粉/乳清蛋白分离凝聚微胶囊结肠靶向递送油基乙醇胺和促进饱腹感激素分泌的结构调控

饮食调节结肠L细胞的饱腹激素胰高血糖素样肽-1 （GLP-1）和YY肽（PYY）的分泌可实现食欲调节和体重控制。本研究以羧甲基淀粉(CMS)/变性乳清分离蛋白（DWPI）凝聚微胶囊（CDCMs）包封油基乙醇酰胺（OEA），构建口服结肠靶向给药系统。此外，单宁酸作为交联剂调节组分之间的相互作用，形成更加致密有序的结构，其粒径范围为12.36 μm - 13.30 μm， OEA负载能力范围为240.34 μg/mL ~ 259.34 μg/mL。随着CL含量的增加，CDCMs在模拟上胃肠道中表现出良好的维持结构完整性的能力，CMS和DWPI的降解率分别从30.53%下降到65.04% - 8.67%和26.33%，OEA的释放率从88.18%下降到20.61%，显示出良好的结肠靶向递送性能。此外，结肠中的CDCM-1发酵增加了肠道细菌的丰度，特别是产生短链脂肪酸（SCFAs）的细菌。此外，OEA的缓释（48h后释放24.34 mg/mL）与CMS/DWPI代谢物（如还原糖、蛋白水解物和SCFAs）协同，有效调节L细胞的GLP-1和PYY分泌，48h时的最高释放量分别为28.03 pg/mL和45.50 pg/mL。因此，制备的cdcm具有成为控制体重的潜在食品的潜力。

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来源期刊

Food Hydrocolloids 工程技术-食品科技

CiteScore

19.90

自引率

14.00%

发文量

871

审稿时长

37 days

期刊介绍： Food Hydrocolloids publishes original and innovative research focused on the characterization, functional properties, and applications of hydrocolloid materials used in food products. These hydrocolloids, defined as polysaccharides and proteins of commercial importance, are added to control aspects such as texture, stability, rheology, and sensory properties. The research's primary emphasis should be on the hydrocolloids themselves, with thorough descriptions of their source, nature, and physicochemical characteristics. Manuscripts are expected to clearly outline specific aims and objectives, include a fundamental discussion of research findings at the molecular level, and address the significance of the results. Studies on hydrocolloids in complex formulations should concentrate on their overall properties and mechanisms of action, while simple formulation development studies may not be considered for publication. The main areas of interest are: -Chemical and physicochemical characterisation Thermal properties including glass transitions and conformational changes- Rheological properties including viscosity, viscoelastic properties and gelation behaviour- The influence on organoleptic properties- Interfacial properties including stabilisation of dispersions, emulsions and foams- Film forming properties with application to edible films and active packaging- Encapsulation and controlled release of active compounds- The influence on health including their role as dietary fibre- Manipulation of hydrocolloid structure and functionality through chemical, biochemical and physical processes- New hydrocolloids and hydrocolloid sources of commercial potential. The Journal also publishes Review articles that provide an overview of the latest developments in topics of specific interest to researchers in this field of activity.