Isolation, identification, pathogenicity and development of a rapid detection technique of Pseudomonas aeruginosa strain GX2021 causing bacterial shell erosion disease in farmed Bellamya aeruginosa

Abstract

Bellamya aeruginosa is an economically important freshwater gastropod. Recently, a bacterial shell erosion disease has frequently occurred in B. aeruginosa farms, and has become an important factor threatening the development of B. aeruginosa aquaculture. To explore the cause of disease, we dissected the diseased B. aeruginosa and isolated a dominant strain named Pseudomonas aeruginosa strain GX2021. The pathogenicity of the isolate GX2021 to B. aeruginosa was determined by a direct challenge experiment. Drug sensitivity experiments revealed that P. aeruginosa strain GX2021 was highly sensitive to ceftriaxone sodium, and moderately sensitive to carbenicillin. A PCR assay for rapid detection and identification of P. aeruginosa based on the DNA sequence of the P. aeruginosa strain GX2021 dnaA gene was developed to detect P. aeruginosa. These findings provided a theoretical foundation and practical guidance for prevention and control of shell erosion disease caused by P. aeruginosa in farmed B. aeruginosa.