Chromosome-level genome assembly of the lemon sole, Microstomus kitt (Pleuronectiformes: Pleuronectidae).

GigaByte (Hong Kong, China) Pub Date : 2025-05-27 eCollection Date: 2025-01-01 DOI:10.46471/gigabyte.156

Marcel Nebenführ, David Prochotta, Maria A Nilsson, Menno J de Jong, Tunca D Yazici, Fabienne Langefeld, Malambo Muloongo, Helena Woköck, Jakob Jilg, Sina C Bender, Marvin M Zangl, Juan-Manuel Ortega Guatame, Kimberley Williams, Moritz Sonnewald, Axel Janke

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Abstract

Background: The lemon sole (Microstomus kitt) is a culinary fish from the family of righteye flounders (Pleuronectidae), inhabiting sandy, shallow offshore grounds of the North Sea, western Baltic Sea, English Channel, Great Britain and Ireland, Bay of Biscay, and coastal waters of Norway.

Findings: Here, we present a chromosome-level genome assembly of the lemon sole. We applied PacBio HiFi sequencing on the PacBio Revio system to generate a highly complete and contiguous reference genome.The resulting assembly has a contig N50 of 17.2 Mbp and a scaffold N50 of 27.2 Mbp. The total assembly length is 628 Mbp, comprising 24 chromosome-length scaffolds. The identification of 99.7% complete BUSCO genes indicates a high level of assembly completeness.

Conclusions: The chromosome-level genome assembly of the lemon sole provides a high-quality reference genome for future population-level genomic analyses of this commercially valuable, edible fish.

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小比目鱼柠檬比目鱼染色体水平的基因组组装。

背景：柠檬比目鱼（Microstomus kitt）是一种食用鱼，属于义鲽科（Pleuronectidae），生活在北海、波罗的海西部、英吉利海峡、大不列颠和爱尔兰、比斯开湾和挪威沿海水域的浅海沙滩上。研究结果：在这里，我们提出了柠檬比目鱼的染色体水平基因组组装。我们在PacBio Revio系统上应用PacBio HiFi测序，生成高度完整和连续的参考基因组。所得到的组装体的N50为17.2 Mbp，支架N50为27.2 Mbp。总组装长度为628 Mbp，由24个染色体长度的支架组成。鉴定出99.7%完整的BUSCO基因，表明具有较高的组装完整性。结论：柠檬比目鱼的染色体水平基因组组装为未来这种具有商业价值的食用鱼类的种群水平基因组分析提供了高质量的参考基因组。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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