Genome-Wide Identification of Quantitative Trait Loci Conferring Adult-Plant Resistance and All-Stage Resistance to Stripe Rust in the Chinese Wheat Landrace Lantian 10.
X L Zhou, Y Q Luo, H Q Li, Y Q Wang, X Li, Q Yao, J F Wang, X J Wang, H Bux, B L Zhang, S Z Yang, Z S Kang, R S Ren
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引用次数: 0
Abstract
Stripe rust, which is caused by Puccinia striiformis f. sp. tritici (Pst), is a devastating foliar disease of wheat that significantly constrains wheat production worldwide. The characterization and utilization of resistance genes represent the most effective, economic, and environmentally friendly strategies for disease control. In this study, the wheat cultivar Lantian 10, which is predominantly resistant to Chinese Pst races, was crossed with the susceptible genotype known as Mingxian 169. The resulting recombinant inbred line population (comprising 152 lines) was evaluated for stripe rust resistance across multiple environment trials. Using the wheat 35K single-nucleotide polymorphism array for genotyping and inclusive composite interval mapping, we identified six stable quantitative trait loci (QTLs) on chromosomes 1A, 1D, 2A, 2B, 2D, and 5B: QYrLT10.swust-1AS, QYrLT10.swust-1DS, QYrLT10.swust-2AS, QYrLT10.swust-2BS, QYrLT10.swust-2DS, and QYrLT10.swust-5BL. These aforementioned QTLs explained at least 18.48 and 19.32% (QYrLT10.swust-1AS), 16.03 and 15.92% (QYrLT10.swust-1DS), 9.55 and 11.34% (QYrLT10.swust-2AS), 9.29 and 11.13% (QYrLT10.swust-2BS), 9.52 and 12.06% (QYrLT10.swust-2DS), and 17.42 and 18.41% (QYrLT10.swust-5BL) of the variations in stripe rust infection type and disease severity, respectively. Based on evaluations of seedling and adult-plant resistance, QYrLT10.swust-1AS and QYrLT10.swust-1DS are speculated to harbor adult-plant resistance genes, whereas the other four QTLs are considered all-stage resistance genes. The identified QTLs and their linked markers will be invaluable for marker-assisted selection in wheat resistance breeding programs.
期刊介绍：
Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.
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