Quantitative PCR for parasitemia monitoring and preemptive therapy in patients with Chagas disease and autoimmune rheumatic disorders undergoing biologic treatment: a case series.

IF 2.3 4区 医学 Q2 PARASITOLOGY
Noemia Barbosa Carvalho, Vera Lucia Teixeira de Freitas, Nadia Emi Aikawa, Lucas Teixeira Vieira, Rita Cristina Bezerra, Adriana Coraccini Tonácio de Proença, Karina Bonfiglioli, Érika Yoshie Shimoda Nakanishi, Hermes Ryoiti Higashino, Maria Aparecida Shikanai-Yasuda
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引用次数: 0

Abstract

Patients with chronic Chagas disease (CD) and autoimmune rheumatic disorders (ARD) receiving immunosuppressive therapy are at risk for CD reactivation (CDR). This study monitored parasitemia over 9-121 months in six patients with CD and ARD using conventional and quantitative PCR and parasitology. Five patients showed parasitemia; two had elevated levels of parEq/mL (49-458.7). One patient received benznidazole with a marked decrease in parasitemia; in the other, treatment was discontinued after 12 days due to toxicity. No CDR occurred. These findings support the need for qPCR standardization for preemptive therapy and suggest that benznidazole may prevent CDR in patients with high parasitemia.

在接受生物治疗的恰加斯病和自身免疫性风湿病患者中进行寄生虫血症监测和先发制人治疗的定量PCR:一个病例系列
接受免疫抑制治疗的慢性恰加斯病(CD)和自身免疫性风湿性疾病(ARD)患者有CD再激活(CDR)的风险。本研究对6例CD和ARD患者进行了为期9-121个月的寄生虫血症监测,采用了常规和定量PCR及寄生虫学方法。5例出现寄生虫血症;2例患者parEq/mL升高(49-458.7)。1例患者接受苯并硝唑治疗后,寄生虫病明显减少;在另一组中,由于毒性,治疗在12天后停止。无话单。这些发现支持了qPCR标准化的必要性,并提示苯并硝唑可能预防高寄生虫血症患者的CDR。
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来源期刊
CiteScore
3.40
自引率
10.00%
发文量
195
审稿时长
3-8 weeks
期刊介绍: The Journal of the Brazilian Society of Tropical Medicine (JBSTM) isan official journal of the Brazilian Society of Tropical Medicine) with open access. It is amultidisciplinary journal that publishes original researches related totropical diseases, preventive medicine, public health, infectious diseasesand related matters. Preference for publication will be given to articlesreporting original observations or researches. The journal has a peer-reviewsystem for articles acceptance and its periodicity is bimonthly. The Journalof the Brazilian Society of Tropical Medicine is published in English.The journal invites to publication Major Articles, Editorials, Reviewand Mini-Review Articles, Short Communications, Case Reports, TechnicalReports, Images in Infectious Diseases, Letters, Supplements and Obituaries.
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