Synthesis of ZIF-67 Nanoparticles for Camel Whey Protein Delivery: Promising Antioxidant, Anti-inflammatory, Anticancer Effects, and Anti-biofilm Activity.
D Hamad, Emad H M Hassanein, Shimaa H Salem, Fatma M Tawfiq, Ahmed M Sayed
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引用次数: 0
Abstract
Camel whey protein (CWP) offers various health benefits, including immune enhancement, anti-inflammatory, anticancer, and antibacterial properties. It also possesses antioxidant activity. However, its limited efficacy and stability restrict its broader application. Metal-organic frameworks (MOFs) are crystalline materials composed of multiple organic groups and metal ions, known for their unique structural properties. In this study, we aimed to synthesize and evaluate the biological activity of a CWP-Co-MOF conjugate. The structural characterization of the synthesized materials was conducted using X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and energy-dispersive X-ray (EDX) analysis. The comparison of the XRD and FTIR patterns of ZIF-67, CWP and CWP-Co-MOF conjugate indicate successful conjugation of CWP with ZIF-67, confirming the structural integrity of the conjugate. The EDX maps further corroborate the effective conjugation of CWP with ZIF-67. The conjugated CWP-MOF nanoparticles (NPs) exhibited promising antioxidant activity, as assessed by the DPPH assay. Furthermore, they showed more potent anti-inflammatory effects in LPS-induced BV2 microglial cells and superior anticancer activity against HepG2 and Caco-2 cell lines, as determined by the MTT assay and flow cytometry, compared to free CWP. Additionally, the CWP-MOF-NPs exhibited enhanced antimicrobial properties and increased efficacy as an anti-biofilm agent against pathogenic bacteria.
骆驼乳清蛋白（CWP）具有多种健康益处，包括增强免疫力、抗炎、抗癌和抗菌特性。它还具有抗氧化活性。但其有限的功效和稳定性限制了其广泛应用。金属有机骨架（mof）是由多种有机基团和金属离子组成的晶体材料，以其独特的结构特性而闻名。本研究旨在合成一种CWP-Co-MOF偶联物并对其生物活性进行评价。利用x射线衍射（XRD）、傅里叶变换红外（FTIR）光谱、扫描电镜（SEM）和能量色散x射线（EDX）分析对合成材料进行了结构表征。ZIF-67、CWP和CWP- co - mof共轭物的XRD和FTIR图对比表明，CWP与ZIF-67成功偶联，证实了该共轭物的结构完整性。EDX图谱进一步证实了CWP与ZIF-67的有效结合。通过DPPH测定，共轭CWP-MOF纳米颗粒（NPs）显示出良好的抗氧化活性。此外，通过MTT实验和流式细胞术，与游离CWP相比，它们对lps诱导的BV2小胶质细胞具有更强的抗炎作用，对HepG2和Caco-2细胞系具有更强的抗癌活性。此外，CWP-MOF-NPs表现出增强的抗菌性能和作为抗生物膜剂对致病菌的功效。
期刊介绍：
Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.
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