Distinct Activation and Functional Features of CD307c+ T Lymphocytes in Peripheral Blood as Diagnostic and Prognostic Markers in Breast Cancer.

IF 4.9 3区 医学 Q2 IMMUNOLOGY
Immunology Pub Date : 2025-06-04 DOI:10.1111/imm.13960
Ziqi Xiong, Zhenxue Li, Zhao Guan, Sen Zhou, Yiming Zhao, Ming Zhao, Xiancan Ma, Yiming Gao, Zhonghui Zhang, Pingzhang Wang, Chen Liu
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引用次数: 0

Abstract

This study aims to investigate the expression and role of CD307c in the breast cancer (BC) microenvironment, T lymphocytes of peripheral blood, particularly in BC patients at various stages, and assess its potential as a clinical diagnostic biomarker. Bioinformatics analysis was performed to investigate CD307c expression. As experimental validation, a total of 54 BC patients and 44 healthy controls (HCs) were enrolled. Flow cytometry was used to analyse CD307c expression in CD4+ and CD8+ T cells, alongside markers of T cell activation and function, such as PD-1, Ki-67, CD25, CD62L, GZMB and GZMK. Ex vivo T cell stimulation with anti-CD3 and anti-CD28 was performed to assess CD307c expression after activation. Statistical analyses, including receiver operating characteristic (ROC) curve analysis, were used to evaluate the diagnostic value of CD307c+ T cell subsets. Bioinformatics analysis revealed that CD307c was significantly upregulated in BC tissues compared to adjacent normal tissues, and that CD307c was mainly expressed in lymphocytes, such as B cells, Tregs, CD8+ T cells and NKs in the tumour microenvironment. In peripheral blood, CD307c expression was significantly higher in CD4+ T cells compared to CD8+ T cells. CD307c+ T cells exhibited elevated levels of Ki-67, PD-1, CD25 and CD62L, indicating increased activation and potential for immune exhaustion. Additionally, CD307c+ CD8+ T cells showed higher expression of granzyme B (GZMB) and granzyme K (GZMK), markers of cytotoxicity. In BC patients, CD307c expression was significantly higher in both CD4+ and CD8+ T cells compared to HCs, and the proportion of CD307c+ cells varied across cancer stages. CD307c expression in T lymphocytes is elevated in early-stage BC. CD307c+ T cells show enhanced activation, suggesting its potential role as a useful biomarker for early BC diagnosis and a potential therapeutic target.

外周血CD307c+ T淋巴细胞作为乳腺癌诊断和预后标志物的独特激活和功能特征
本研究旨在探讨CD307c在乳腺癌(BC)微环境、外周血T淋巴细胞中的表达和作用,特别是在不同阶段的BC患者中,并评估其作为临床诊断生物标志物的潜力。生物信息学分析CD307c的表达情况。作为实验验证,共入组54例BC患者和44例健康对照(hc)。流式细胞术分析CD307c在CD4+和CD8+ T细胞中的表达,以及T细胞活化和功能标志物PD-1、Ki-67、CD25、CD62L、GZMB和GZMK的表达。用抗cd3和抗cd28刺激体外T细胞,评估活化后CD307c的表达。采用统计学分析,包括受试者工作特征(ROC)曲线分析,评价CD307c+ T细胞亚群的诊断价值。生物信息学分析显示,与邻近正常组织相比,CD307c在BC组织中表达显著上调,CD307c主要表达于肿瘤微环境中的淋巴细胞,如B细胞、Tregs细胞、CD8+ T细胞和NKs细胞。在外周血中,CD307c在CD4+ T细胞中的表达明显高于CD8+ T细胞。CD307c+ T细胞表现出Ki-67、PD-1、CD25和CD62L水平升高,表明激活增加和免疫衰竭的可能性。此外,CD307c+ CD8+ T细胞中颗粒酶B (GZMB)和颗粒酶K (GZMK)的表达也较高。在BC患者中,CD307c在CD4+和CD8+ T细胞中的表达明显高于hcc,并且CD307c+细胞的比例在不同的癌症分期中有所不同。CD307c在早期BC患者T淋巴细胞中的表达升高。CD307c+ T细胞活化增强,提示其作为早期BC诊断的有用生物标志物和潜在治疗靶点的潜在作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Immunology
Immunology 医学-免疫学
CiteScore
11.90
自引率
1.60%
发文量
175
审稿时长
4-8 weeks
期刊介绍: Immunology is one of the longest-established immunology journals and is recognised as one of the leading journals in its field. We have global representation in authors, editors and reviewers. Immunology publishes papers describing original findings in all areas of cellular and molecular immunology. High-quality original articles describing mechanistic insights into fundamental aspects of the immune system are welcome. Topics of interest to the journal include: immune cell development, cancer immunology, systems immunology/omics and informatics, inflammation, immunometabolism, immunology of infection, microbiota and immunity, mucosal immunology, and neuroimmunology. The journal also publishes commissioned review articles on subjects of topical interest to immunologists, and commissions in-depth review series: themed sets of review articles which take a 360° view of select topics at the heart of immunological research.
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