Correlation between interleukins in aqueous humor and vitreous humor of vitreoretinal lymphoma patients.

IF 4 1区医学 Q1 OPHTHALMOLOGY

Eye and Vision Pub Date : 2025-06-05 DOI:10.1186/s40662-025-00438-0

Yurun Liu, Xinyi Zhou, Kaiyu Zhang, Shixue Liu, Ruiwen Li, Yifan Gong, Zhujian Wang, Tingting Jiang, Ting Zhang, Gezhi Xu, Junxiang Gu, Qing Chang

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引用次数: 0

Abstract

Background: Interleukin detection is helpful in screening vitreoretinal lymphoma (VRL). However, the levels of interleukin in aqueous humor (AqH) can be abnormally low in some cases, leading to underdiagnosis of VRL merely dependent on AqH. The purpose of this study was to investigate the correlation of interleukins between paired AqH and vitreous humor (VH) samples in VRL cases, and to explore potential factors affecting interleukin levels and diagnostic parameters.

Methods: This was a case series study. Reviewed were consecutive biopsy-proven B-cell VRL cases of which adequate paired AqH and VH samples were obtained for the measurement of interleukin 10 (IL-10) and interleukin 6 (IL-6). The correlations of IL-10 and IL-6 between AqH and VH were analyzed. Influences of clinical manifestations on IL levels and positive rates of IL-related parameters in AqH and VH were evaluated, which included AqH IL-10 > 30 pg/mL, VH IL-10 > 65 pg/mL, IL-10/IL-6 ratio > 1, and Interleukin Score for Intraocular Lymphoma Diagnosis (ISOLD) > 0 in both the AqH and VH.

Results: Seventy-four eyes of 64 patients with VRL were included. IL-10 in VH was significantly higher than in AqH (median: 1159.77 vs. 225.74 pg/mL, P < 0.001). For both IL-10 and IL-6, the AqH concentrations were positively correlated with VH concentrations in the form of power functions (P < 0.001 and P < 0.001, respectively). The positive rate of AqH IL-10/IL-6 > 1 (77%) was lower than that of VH IL-10 > 65 pg/mL (91%), VH IL-10/IL-6 > 1 (89%) and VH ISOLD > 0 (91%). Eyes without intraretinal infiltration tended to have lower IL-10 levels in the AqH and VH (median: 141.08 pg/mL vs. 449.10 pg/mL, 825.48 pg/mL vs. 2285.77 pg/mL; P = 0.001 and P < 0.001, respectively), and lower positive rates of AqH IL-10 > 30 pg/mL (78% vs. 97%, P = 0.018) and AqH ISOLD > 0 (76% vs. 97%, P = 0.033).

Conclusions: IL-10/IL-6 in AqH may not be as sensitive as the parameters (including IL-10, IL-10/IL-6 and ISOLD) in VH for VRL screening. Cases without intraretinal involvement were less likely to be positive for IL-10 > 30 pg/mL and ISOLD > 0 in AqH; the possibility of VRL should be ruled out more cautiously in these cases.

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玻璃体视网膜淋巴瘤患者房水和玻璃体中白细胞介素的相关性研究。

背景：白细胞介素检测有助于筛查玻璃体视网膜淋巴瘤（VRL）。然而，房水中白细胞介素（AqH）的水平在某些情况下可能异常低，导致仅依赖AqH的VRL诊断不足。本研究旨在探讨VRL患者配对AqH与玻璃体房颤（VH）样本间白细胞介素的相关性，并探讨影响白细胞介素水平和诊断参数的潜在因素。方法：采用病例系列研究。回顾了连续活检证实的b细胞VRL病例，这些病例获得了足够的配对AqH和VH样本，用于测量白细胞介素10 （IL-10）和白细胞介素6 （IL-6）。分析AqH与VH之间IL-10、IL-6的相关性。评估临床表现对AqH和VH中IL水平和IL相关参数阳性率的影响，包括AqH中IL-10 > 30 pg/mL， VH中IL-10 > 65 pg/mL， IL-10/IL-6比值> 1，以及眼内淋巴瘤诊断白细胞介素评分(ISOLD) > 0。结果：纳入64例VRL患者74只眼。VH组IL-10显著高于AqH组（中位数：1159.77 vs. 225.74 pg/mL）， p1（77%）低于VH组IL-10 > 65 pg/mL（91%）、VH组IL-10/IL-6 >(89%)和VH组ISOLD >(91%)。没有视网膜内浸润的眼睛，AqH和VH的IL-10水平往往较低(中位数：141.08 pg/mL vs 449.10 pg/mL, 825.48 pg/mL vs 2285.77 pg/mL；P = 0.001和P 30 pg / mL(78%比97%,P = 0.018)和AqH ISOLD > 0(76%比97%,P = 0.033)。结论：在筛选VRL时，AqH中IL-10/IL-6可能不如VH参数（包括IL-10、IL-10/IL-6和ISOLD）灵敏。无肠内受累的患者在AqH中IL-10 > 30 pg/mL和ISOLD >阳性的可能性较小；在这些情况下，应更谨慎地排除VRL的可能性。

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来源期刊

Eye and Vision OPHTHALMOLOGY-

CiteScore

8.60

自引率

2.40%

发文量

审稿时长

15 weeks

期刊介绍： Eye and Vision is an open access, peer-reviewed journal for ophthalmologists and visual science specialists. It welcomes research articles, reviews, methodologies, commentaries, case reports, perspectives and short reports encompassing all aspects of eye and vision. Topics of interest include but are not limited to: current developments of theoretical, experimental and clinical investigations in ophthalmology, optometry and vision science which focus on novel and high-impact findings on central issues pertaining to biology, pathophysiology and etiology of eye diseases as well as advances in diagnostic techniques, surgical treatment, instrument updates, the latest drug findings, results of clinical trials and research findings. It aims to provide ophthalmologists and visual science specialists with the latest developments in theoretical, experimental and clinical investigations in eye and vision.