{"title":"Production of functional recombinant antibodies in Dictyostelium discoideum.","authors":"Cyril Guilhen, Tania Jauslin","doi":"10.1186/s13104-025-07314-z","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Recombinant antibodies are essential reagents for diagnostics, research, and therapy. Numerous production methods have been developed, each of them with its strengths and weaknesses. In this study we evaluated the ability of Dictyostelium discoideum cells to produce and secrete functional antibodies.</p><p><strong>Results: </strong>Three recombinant antibodies targeting tubulin, CISD1 or CD8β proteins, respectively, were successfully produced and secreted by D. discoideum cells. Electrophoretic analysis of these antibodies revealed a degradation product, resulting from proteolytic cleavage at the linker peptide connecting the scFv portion to the Fc fragment. Removal of this linker suppressed the proteolytic cleavage. Finally, immunofluorescence analysis confirmed that all three antibodies recognized their target antigen in a specific manner. This study represents the first demonstration that functional recombinant antibodies can be produced in D. discoideum cells.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"246"},"PeriodicalIF":1.6000,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12139333/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Research Notes","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s13104-025-07314-z","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: Recombinant antibodies are essential reagents for diagnostics, research, and therapy. Numerous production methods have been developed, each of them with its strengths and weaknesses. In this study we evaluated the ability of Dictyostelium discoideum cells to produce and secrete functional antibodies.
Results: Three recombinant antibodies targeting tubulin, CISD1 or CD8β proteins, respectively, were successfully produced and secreted by D. discoideum cells. Electrophoretic analysis of these antibodies revealed a degradation product, resulting from proteolytic cleavage at the linker peptide connecting the scFv portion to the Fc fragment. Removal of this linker suppressed the proteolytic cleavage. Finally, immunofluorescence analysis confirmed that all three antibodies recognized their target antigen in a specific manner. This study represents the first demonstration that functional recombinant antibodies can be produced in D. discoideum cells.
BMC Research NotesBiochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
3.60
自引率
0.00%
发文量
363
审稿时长
15 weeks
期刊介绍：
BMC Research Notes publishes scientifically valid research outputs that cannot be considered as full research or methodology articles. We support the research community across all scientific and clinical disciplines by providing an open access forum for sharing data and useful information; this includes, but is not limited to, updates to previous work, additions to established methods, short publications, null results, research proposals and data management plans.
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