Comparative analyses of serum parameters between nontransgenic and transgenic male offspring chickens expressing the 3D8 single-chain variable fragment gene and.

Abstract

Objective: Transgenic (TG) animals offer significant potential for diverse applications but may pose risks if the impact of transgene expression on health and physiological parameters is not thoroughly assessed. This study aims to evaluate the effects of 3D8 scFv gene expression on male TG chickens, focusing on key biological markers, mortality, and growth.

Methods: Serum samples were collected at 14 weeks of age from male TG and non-TG chickens for comprehensive analysis of serum biochemistry, sex hormones, and cytokine profiles. Mortality and growth were monitored over a 34-week period to assess long-term effects. Statistical comparisons were conducted between TG and non-TG groups to identify significant differences.

Results: The results revealed that male non-TG chickens exhibited significantly higher levels of ALT, IGF-1, IFN-γ, and IL-4 compared to TG chickens (p<0.05). However, no significant differences in survival or growth were observed between TG and non-TG groups (p>0.05). These findings are consistent with previous studies on female TG offspring, which reported no significant impact of 3D8 gene expression on mortality, growth, or productivity.

Conclusion: 3D8 scFv gene expression in male TG chickens does not adversely affect mortality, growth, or serum parameters, indicating that the transgene does not have detrimental effects on these critical metrics in male chickens. These findings support the safety and viability of 3D8 gene expression in TG animals.