The mechanism of promoting angiogenesis after cerebral infarction by scalp acupuncture based on MATN2/WNT3a/β-catenin.

IF 2.9 4区 生物学 Q3 CELL BIOLOGY
Qian Zhang, Shengwang Han, Yi Zheng, Jiayuan Li, Le Ma, Jinlang Tan, Xingyu Kang, Rui Gong, Siyu Chen, Shuai Shi
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引用次数: 0

Abstract

Scalp acupuncture, a therapeutic modality derived from traditional acupuncture theory, has been widely applied in clinical practice for cerebral infarction management in China. In this study, a middle cerebral artery occlusion (MCAO) rat model was established. Therapeutic efficacy was evaluated through multidimensional approaches: (1) Neurological functional assessment using modified Neurological Severity Score (mNSS); (2) Quantitative infarct volume measurement via 2,3,5-triphenyltetrazolium chloride (TTC) staining; (3) Histopathological analysis through hematoxylin-eosin (HE) staining. Additional analytical techniques including transmission electron microscopy (TEM) and immunofluorescence staining were employed to investigate the expression patterns of MATN2, WNT3a, β-catenin, and CD34 biomarkers, elucidating the potential mechanisms underlying scalp acupuncture-induced angiogenesis post-cerebral infarction in animal models. Key findings included: marked reduction in mNSS scores at 1-day and 7-day post-intervention intervals (P < 0.01); substantial decrease in cerebral infarction volume quantified by TTC staining (P < 0.05); enhanced neuronal density and preserved cytoarchitectural integrity in peri-infarct regions observed through HE staining. Ultrastructural analysis via TEM revealed notable improvements in microvascular endothelial cell (EC) morphology and intercellular junctions. Immunofluorescence quantification showed upregulated expression of pro-angiogenic factors MATN2, WNT3a, and β-catenin, accompanied by increased CD34 + microvascular density (P < 0.01). This study provides comprehensive experimental evidence that scalp acupuncture administration facilitates post-ischemic angiogenesis, suggest potential therapeutic applications for stroke rehabilitation.

基于MATN2/WNT3a/β-catenin的头皮针刺促进脑梗死后血管生成的机制
头皮针刺作为一种源自传统针灸理论的治疗方式,在中国已广泛应用于脑梗死的临床治疗。建立大脑中动脉闭塞(MCAO)大鼠模型。通过多维度评价治疗效果:(1)采用改良神经功能评分法(mNSS)进行神经功能评价;(2)通过2,3,5-三苯基四氯化氮(TTC)染色定量测定梗死体积;(3)苏木精-伊红(HE)染色进行组织病理学分析。采用透射电镜(TEM)和免疫荧光染色等分析技术,研究了MATN2、WNT3a、β-catenin和CD34生物标志物的表达模式,阐明了头皮针刺诱导脑梗死后血管生成的潜在机制。主要发现包括:干预后1天和7天的mNSS评分显著降低(P
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来源期刊
Journal of Molecular Histology
Journal of Molecular Histology 生物-细胞生物学
CiteScore
5.90
自引率
0.00%
发文量
68
审稿时长
1 months
期刊介绍: The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.
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