Effects of biological carrier optimization on hydrogen production and biofilm formation in anaerobic circulating fluidized bed reactor via dark fermentation

IF 8.1 2区工程技术 Q1 CHEMISTRY, PHYSICAL

International Journal of Hydrogen Energy Pub Date : 2025-06-05 DOI:10.1016/j.ijhydene.2025.06.019

Yufei Fu , Yangfan Song , Hongwei Chen , Hao Chen , Yanmin Li , Qianyun Wu

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Abstract

Promoting the adhesion of microorganisms to carriers is conducive to facilitating the hydrogen production of dark fermentation in biofilm reactors. Five methods for enhancing the attachment of microorganisms to carriers were proposed to improve the hydrogen production performance of novel biofilm reactor, anaerobic circulating fluidized bed reactor (ACFBR). In this study, acid, activated carbon, graphite, silane coupling agent and quaternary ammonium salt were used to optimize polyamide 6 (PA6), and compared with untreated PA6 carrier to explore the influence of carrier optimization on biological hydrogen production, biofilm formation and wastewater treatment in ACFBR. Escherichia coli and synthetic wastewater were used in continuous fermentation experiments with a hydraulic retention time (HRT) of 4 h. The results showed that acid treatment developed maximum reactor biomass (38.95 g). Silane treatment achieved greatest average biofilm thickness (4.12 μm) and density (0.579 g/cm³). In terms of hydrogen production and wastewater treatment, acid treatment achieved peak hydrogen yield (0.921 mol-H₂/mol-glucose), hydrogen production rate (0.138 L-H₂/(L·h)), and COD removal efficiency (32.15 %).

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生物载体优化对厌氧循环流化床暗发酵产氢及生物膜形成的影响

促进微生物与载体的粘附有利于促进生物膜反应器暗发酵产氢。为提高新型生物膜反应器厌氧循环流化床反应器（ACFBR）的产氢性能，提出了五种增强微生物与载体附着的方法。本研究采用酸、活性炭、石墨、硅烷偶联剂和季铵盐对聚酰胺6 （PA6）进行优化，并与未处理的PA6载体进行对比，探讨载体优化对ACFBR生物制氢、生物膜形成和废水处理的影响。利用大肠杆菌和合成废水进行连续发酵实验，水力停留时间（HRT）为4 h。结果表明，酸处理产生的反应器生物量最大（38.95 g）。硅烷处理获得了最大的平均生物膜厚度（4.12 μm）和密度（0.579 g/cm3）。在产氢和废水处理方面，酸处理的产氢率最高（0.921 mol-H2/mol-葡萄糖），产氢率最高（0.138 L- h2 /(L·h)）， COD去除率最高（32.15%）。

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来源期刊

International Journal of Hydrogen Energy 工程技术-环境科学

CiteScore

13.50

自引率

25.00%

发文量

3502

审稿时长

60 days

期刊介绍： The objective of the International Journal of Hydrogen Energy is to facilitate the exchange of new ideas, technological advancements, and research findings in the field of Hydrogen Energy among scientists and engineers worldwide. This journal showcases original research, both analytical and experimental, covering various aspects of Hydrogen Energy. These include production, storage, transmission, utilization, enabling technologies, environmental impact, economic considerations, and global perspectives on hydrogen and its carriers such as NH3, CH4, alcohols, etc. The utilization aspect encompasses various methods such as thermochemical (combustion), photochemical, electrochemical (fuel cells), and nuclear conversion of hydrogen, hydrogen isotopes, and hydrogen carriers into thermal, mechanical, and electrical energies. The applications of these energies can be found in transportation (including aerospace), industrial, commercial, and residential sectors.