Preliminary Exploration of Paper-based ELISA with Recombinant Thioredoxin Peroxidase-1 for Detection of Human Schistosomiasis.

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2025-06-04 DOI:10.1007/s11686-025-01069-0

John Matthew F Gicom, Alonna Leanne L Egaran, Edric John S Follante, Mariah Kaye R Ebora, Christine Marianne G Dapal, Katrina Theresa M Balboa, Klyde Irene M Ligot, Jose Ma M Angeles

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引用次数: 0

Abstract

Purpose: This study addresses these limitations by evaluating a field-deployable diagnostic alternative, a paper-based enzyme-linked immunosorbent assay (ELISA) incorporating recombinant thioredoxin peroxidase-1 (rSjTPx-1).

Methods and results: The study focused on optimizing the antigen concentration and serum dilution parameters to enhance assay performance. Subsequently, the diagnostic utility of the optimized rSjTPx-1 paper-based ELISA was evaluated using archived serum samples from individuals in a schistosomiasis-endemic region of the Philippines. These samples had been previously classified using the conventional rSjTPx-1 ELISA as seronegative (n = 3), seropositive with low optical density (OD) values (n = 3), and seropositive with high OD values (n = 3). Compared to the conventional rSjTPx-1-ELISA, the paper-based format demonstrated a sensitivity of 83%, specificity of 100%, and a kappa value of 0.769, indicating substantial agreement.

Conclusion: Although slightly less sensitive than the conventional rSjTPx-1-ELISA, the paper-based format offers significant advantages in terms of portability, ease of use, and suitability for resource-limited settings. These results suggest that the rSjTPx-1 paper-based ELISA could be an accessible diagnostic tool for S. japonicum in endemic regions.

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重组硫氧还蛋白过氧化物酶-1纸基ELISA检测人血吸虫病的初步探索。

目的：本研究通过评估一种现场可部署的诊断替代方案，一种含有重组硫氧还蛋白过氧化物酶-1 （rSjTPx-1）的纸质酶联免疫吸附试验（ELISA）来解决这些局限性。方法与结果：优化抗原浓度和血清稀释参数，提高检测效果。随后，利用菲律宾血吸虫病流行区个体的存档血清样本，评估了优化后的rSjTPx-1纸基ELISA的诊断效用。这些样本先前使用传统的rSjTPx-1 ELISA法分为血清阴性（n = 3）、低光密度（OD）值血清阳性（n = 3）和高光密度值血清阳性（n = 3）。与传统的rsjtpx -1 elisa相比，纸质格式的灵敏度为83%，特异性为100%，kappa值为0.769，两者具有较好的一致性。结论：虽然灵敏度略低于传统的rsjtpx -1 elisa，但基于纸张的格式在可移植性、易用性和适合资源有限的环境方面具有显著优势。这些结果提示rSjTPx-1纸本ELISA可作为日本血吸虫流行地区的一种简便易行的诊断工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.